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dc.contributor.authorSiddharth, Saket
dc.contributor.authorVittal, Ravishankar Rai
dc.contributor.authorWink, Joachim
dc.contributor.authorSteinert, Michael
dc.date.accessioned2020-02-25T14:45:24Z
dc.date.available2020-02-25T14:45:24Z
dc.date.issued2020-02-07
dc.identifier.citationMicroorganisms. 2020 Feb 7;8(2). pii: microorganisms8020225. doi: 10.3390/microorganisms8020225.en_US
dc.identifier.issn2076-2607
dc.identifier.pmid32046111
dc.identifier.doi10.3390/microorganisms8020225
dc.identifier.urihttp://hdl.handle.net/10033/622168
dc.description.abstractThe search for novel bioactive metabolites continues to be of much importance around the world for pharmaceutical, agricultural, and industrial applications. Actinobacteria constitute one of the extremely interesting groups of microorganisms widely used as important biological contributors for a wide range of novel secondary metabolites. This study focused on the assessment of antimicrobial and antioxidant activity of crude extracts of actinobacterial strains. Western Ghats of India represents unique regions of biologically diverse areas called "hot spots". A total of 32 isolates were obtained from soil samples of different forest locations of Bisle Ghat and Virjapet situated in Western Ghats of Karnataka, India. The isolates were identified as species of Streptomyces, Nocardiopsis, and Nocardioides by cultural, morphological, and molecular studies. Based on preliminary screening, seven isolates were chosen for metabolites extraction and to determine antimicrobial activity qualitatively (disc diffusion method) and quantitatively (micro dilution method) and scavenging activity against DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS(2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radicals. Crude extracts of all seven isolates exhibited fairly strong antibacterial activity towards MRSA strains (MRSA ATCC 33591, MRSA ATCC NR-46071, and MRSA ATCC 46171) with MIC varying from 15.62 to 125 μg/mL, whereas showed less inhibition potential towards Gram-negative bacteria Salmonella typhi (ATCC 25241) and Escherichia coli (ATCC 11775) with MIC of 125-500 μg/mL. The isolates namely S1A, SS5, SCA35, and SCA 11 inhibited Fusariummoniliforme (MTCC 6576) to a maximum extent with MIC ranging from 62.5 to 250 μg/mL. Crude extract of SCA 11 and SCA 13 exhibited potent scavenging activities against DPPH and ABTS radicals. The results from this study suggest that actinobacterial strains of Western Ghats are an excellent source of natural antimicrobial and antioxidant compounds. Further research investigations on purification, recovery, and structural characterization of the active compounds are to be carried out.en_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectMRSAen_US
dc.subjectWestern Ghatsen_US
dc.subjectactinobacteriaen_US
dc.subjectantimicrobialen_US
dc.subjectantioxidantsen_US
dc.subjectdiversityen_US
dc.titleDiversity and Bioactive Potential of Actinobacteria from Unexplored Regions of Western Ghats, India.en_US
dc.typeArticleen_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalMicroorganismsen_US
refterms.dateFOA2020-02-25T14:45:25Z
dc.source.journaltitleMicroorganisms


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