Identification and Heterologous Expression of the Albucidin Gene Cluster from the Marine Strain Subsp. NRRL B-24108.
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Authors
Myronovskyi, MaksymRosenkränzer, Birgit
Stierhof, Marc
Petzke, Lutz
Seiser, Tobias
Luzhetskyy, Andriy
Issue Date
2020-02-10
Metadata
Show full item recordAbstract
Herbicides with new modes of action and safer toxicological and environmental profiles are needed to manage the evolution of weeds that are resistant to commercial herbicides. The unparalleled structural diversity of natural products makes these compounds a promising source for new herbicides. In 2009, a novel nucleoside phytotoxin, albucidin, with broad activity against grass and broadleaf weeds was isolated from a strain of Streptomyces albus subsp. chlorinus NRRL B-24108. Here, we report the identification and heterologous expression of the previously uncharacterized albucidin gene cluster. Through a series of gene inactivation experiments, a minimal set of albucidin biosynthetic genes was determined. Based on gene annotation and sequence homology, a model for albucidin biosynthesis was suggested. The presented results enable the construction of producer strains for a sustainable supply of albucidin for biological activity studies.Citation
Microorganisms. 2020 Feb 10;8(2). pii: microorganisms8020237. doi: 10.3390/microorganisms8020237.Affiliation
HIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1 66123 Saarbrücken, Germany.Publisher
MDPIJournal
MicroorganismsPubMed ID
32050690Type
ArticleLanguage
enISSN
2076-2607ae974a485f413a2113503eed53cd6c53
10.3390/microorganisms8020237
Scopus Count
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