A comprehensive and comparative phenotypic analysis of the collaborative founder strains identifies new and known phenotypes.
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Authors
Kollmus, HeikeFuchs, Helmut
Lengger, Christoph
Haselimashhadi, Hamed
Bogue, Molly A
Östereicher, Manuela A
Horsch, Marion
Adler, Thure
Aguilar-Pimentel, Juan Antonio
Amarie, Oana Veronica
Becker, Lore
Beckers, Johannes
Calzada-Wack, Julia
Garrett, Lillian
Hans, Wolfgang
Hölter, Sabine M
Klein-Rodewald, Tanja
Maier, Holger
Mayer-Kuckuk, Philipp
Miller, Gregor
Moreth, Kristin
Neff, Frauke
Rathkolb, Birgit
Rácz, Ildikó
Rozman, Jan
Spielmann, Nadine
Treise, Irina
Busch, Dirk
Graw, Jochen
Klopstock, Thomas
Wolf, Eckhard
Wurst, Wolfgang
Yildirim, Ali Önder
Mason, Jeremy
Torres, Arturo
Balling, Rudi
Mehaan, Terry
Gailus-Durner, Valerie
Schughart, Klaus
Hrabě de Angelis, Martin
Issue Date
2020-02-14
Metadata
Show full item recordAbstract
Myxococcus xanthus DK1622 is known as a proficient producer of different kinds of secondary metabolites (SM) with various biological activities, including myxovirescin A, myxalamide A, myxochromide A and DKxanthene. Low production of SM in the wild type bacteria makes searching for production optimization methods highly desirable. Identification and induction of endogenous key molecular feature(s) regulating the production level of the metabolites remain promising, while heterologous expression of the biosynthetic genes is not always efficient because of various complicating factors including codon usage bias. This study established proteomic and molecular approaches to elucidate the regulatory roles of the ROK regulatory protein in the modification of secondary metabolite biosynthesis. Interestingly, the results revealed that rok inactivation significantly reduced the production of the SM and also changed the motility in the bacteria. Electrophoretic mobility shift assay using purified ROK protein indicated a direct enhancement of the promoters encoding transcription of the DKxanthene, myxochelin A, and myxalamide A biosynthesis machinery. Comparative proteomic analysis by two-dimensional fluorescence difference in-gel electrophoresis (2D-DIGE) was employed to identify the protein profiles of the wild type and rok mutant strains during early and late logarithmic growth phases of the bacterial culture. Resulting data demonstrated overall 130 differently altered proteins by the effect of the rok gene mutation, including putative proteins suspected to be involved in transcriptional regulation, carbohydrate metabolism, development, spore formation, and motility. Except for a slight induction seen in the production of myxovirescin A in a rok over-expression background, no changes were found in the formation of the other SM. From the outcome of our investigation, it is possible to conclude that ROK acts as a pleiotropic regulator of secondary metabolite formation and development in M. xanthus, while its direct effects still remain speculative. More experiments are required to elucidate in detail the variable regulation effects of the protein and to explore applicable approaches for generating valuable SM in this bacterium.Citation
Mammalian genome : official journal of the International Mammalian Genome Society JID - 9100916.Affiliation
HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.Publisher
SpringerJournal
Mammalian GenomePubMed ID
32060626Type
ArticleLanguage
enISSN
1432-1777ae974a485f413a2113503eed53cd6c53
10.1007/s00335-020-09827-3
Scopus Count
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- Creative Commons
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International
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