Lamellipodin tunes cell migration by stabilizing protrusions and promoting adhesion formation.
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Dimchev et al.pdf
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2021-02-24
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Authors
Dimchev, GeorgiAmiri, Behnam
Humphries, Ashley C
Schaks, Matthias
Dimchev, Vanessa
Stradal, Theresia E B
Faix, Jan
Krause, Matthias
Way, Michael
Falcke, Martin
Rottner, Klemens
Issue Date
2020-02-24
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Show full item recordAbstract
Efficient migration on adhesive surfaces involves the protrusion of lamellipodial actin networks and their subsequent stabilization by nascent adhesions. The actin binding protein lamellipodin (Lpd) is thought to play a critical role in lamellipodium protrusion, by delivering Ena/VASP proteins onto the growing plus ends of actin filaments and by interacting with the WAVE regulatory complex (WRC), an activator of the Arp2/3 complex, at the leading edge. Using B16-F1 melanoma cell lines, we demonstrate that genetic ablation of Lpd compromises protrusion efficiency and coincident cell migration without altering essential parameters of lamellipodia, including their maximal rate of forward advancement and actin polymerization. We also confirmed lamellipodia and migration phenotypes with CRISPR/Cas9-mediated Lpd knockout Rat2 fibroblasts, excluding cell type-specific effects. Moreover, computer-aided analysis of cell edge morphodynamics on B16-F1 cell lamellipodia revealed that loss of Lpd correlates with reduced temporal protrusion maintenance as a prerequisite of nascent adhesion formation. We conclude that Lpd optimizes protrusion and nascent adhesion formation by counteracting frequent, chaotic retraction and membrane ruffling.Citation
J Cell Sci. 2020 Feb 24. pii: jcs.239020. doi: 10.1242/jcs.239020.Affiliation
HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.Publisher
The company of biologistsJournal
Journal of cell sciencePubMed ID
32094266Type
ArticleLanguage
enISSN
1477-9137ae974a485f413a2113503eed53cd6c53
10.1242/jcs.239020
Scopus Count
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