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dc.contributor.authorLicht, Mathieu K
dc.contributor.authorNuss, Aaron M
dc.contributor.authorVolk, Marcel
dc.contributor.authorKonzer, Anne
dc.contributor.authorBeckstette, Michael
dc.contributor.authorBerghoff, Bork A
dc.contributor.authorKlug, Gabriele
dc.date.accessioned2020-03-06T10:01:57Z
dc.date.available2020-03-06T10:01:57Z
dc.date.issued2020-02-19
dc.identifier.citationMicroorganisms. 2020 Feb 19;8(2). pii: microorganisms8020283. doi: 10.3390/microorganisms8020283.en_US
dc.identifier.issn2076-2607
dc.identifier.pmid32093084
dc.identifier.doi10.3390/microorganisms8020283
dc.identifier.urihttp://hdl.handle.net/10033/622190
dc.description.abstractPhotosynthetic bacteria have to deal with the risk of photooxidative stress that occurs in presence of light and oxygen due to the photosensitizing activity of (bacterio-) chlorophylls. Facultative phototrophs of the genus Rhodobacter adapt the formation of photosynthetic complexes to oxygen and light conditions, but cannot completely avoid this stress if environmental conditions suddenly change. R. capsulatus has a stronger pigmentation and faster switches to phototrophic growth than R. sphaeroides. However, its photooxidative stress response has not been investigated. Here, we compare both species by transcriptomics and proteomics, revealing that proteins involved in oxidation-reduction processes, DNA, and protein damage repair play pivotal roles. These functions are likely universal to many phototrophs. Furthermore, the alternative sigma factors RpoE and RpoHII are induced in both species, even though the genetic localization of the rpoE gene, the RpoE protein itself, and probably its regulon, are different. Despite sharing the same habitats, our findings also suggest individual strategies. The crtIB-tspO operon, encoding proteins for biosynthesis of carotenoid precursors and a regulator of photosynthesis, and cbiX, encoding a putative ferrochelatase, are induced in R. capsulatus. This specific response might support adaptation by maintaining high carotenoid-to-bacteriochlorophyll ratios and preventing the accumulation of porphyrin-derived photosensitizers.en_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectRhodobacter capsulatusen_US
dc.subjectRhodobacter sphaeroidesen_US
dc.subjectphotooxidative stressen_US
dc.subjectproteomicsen_US
dc.subjectstress defenseen_US
dc.subjecttranscriptomicsen_US
dc.titleAdaptation to Photooxidative Stress: Common and Special Strategies of the Alphaproteobacteria Rhodobacter sphaeroides and Rhodobacter capsulatus .en_US
dc.typeArticleen_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalMicoorganismsen_US
refterms.dateFOA2020-03-06T10:01:57Z
dc.source.journaltitleMicroorganisms


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