Characterization of a transcriptional TPP riboswitch in the human pathogen Neisseriameningitidis.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Materne, Solange Lise
MetadataShow full item record
AbstractIncreasing evidence has demonstrated that regulatory RNA elements such as riboswitches (RS) play a pivotal role in the fine-tuning of bacterial gene expression. In this study, we investigated and characterized a novel transcriptional thiamine pyrophosphate (TPP) RS in the obligate human pathogen N. meningitidis MC58 (serogroup B). This RS is located in the 5´ untranslated region upstream of thiC gene, encoding a protein involved in TPP biosynthesis, an essential cofactor for all living beings. Primer extension revealed the transcriptional start site of thiC. Northern blot analysis of thiC mRNA and reporter gene studies confirmed the presence of an active TPP-sensing RS. Expression patterns of the wild-type RS and site-specific mutants showed that it is an OFF switch that controls transcription elongation of thiC mRNA. Interestingly, the regulatory mechanism of the meningococcal thiC RS resembles the Gram-positive Bacillus subtilis thiC RS rather than the Gram-negative Escherichia coli thiC RS. Therefore, the meningococcal thiC RS represents a rare example of transcriptional RS in a Gram-negative bacterium. We further observed that the RS is actively involved in modulating gene expression in response to different growth media and to supplemented bacterial and eukaryotic cell lysates as possible sources of nutrients in the nasopharynx. Our results suggest that RS-mediated gene regulation could influence meningococcal fitness, through the fine-tuning of biosynthesis and scavenging of nutrients and cofactors, such as thiamine.
CitationRNA Biol. 2020 Feb 20:1-13. doi: 10.1080/15476286.2020.1727188.
AffiliationHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.
PublisherTaylor & Francis
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International
- Identification and characterisation of thiamine pyrophosphate (TPP) riboswitch in Elaeis guineensis.
- Authors: Subki A, Ho CL, Ismail NFN, Zainal Abidin AA, Balia Yusof ZN
- Issue date: 2020
- Role of a hairpin-stabilized pause in the <i>Escherichia coli thiC</i> riboswitch function.
- Authors: Chauvier A, Nadon JF, Grondin JP, Lamontagne AM, Lafontaine DA
- Issue date: 2019 Aug
- The riboswitch regulates a thiamine pyrophosphate ABC transporter of the oral spirochete Treponema denticola.
- Authors: Bian J, Shen H, Tu Y, Yu A, Li C
- Issue date: 2011 Aug
- Structural basis for gene regulation by a thiamine pyrophosphate-sensing riboswitch.
- Authors: Serganov A, Polonskaia A, Phan AT, Breaker RR, Patel DJ
- Issue date: 2006 Jun 29
- Conformational Dynamics of thiM Riboswitch To Understand the Gene Regulation Mechanism Using Markov State Modeling and the Residual Fluctuation Network Approach.
- Authors: Kesherwani M, N H V K, Velmurugan D
- Issue date: 2018 Aug 27