Structure of a Protein-RNA Complex by Solid-State NMR Spectroscopy.
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Ahmed, Marchanka and Carlomagno.pdf
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Issue Date
2020-02-05
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Solid-state NMR (ssNMR) is applicable to high molecular-weight (MW) protein assemblies in a non-amorphous precipitate. The technique yields atomic resolution structural information on both soluble and insoluble particles without limitations of MW or requirement of crystals. Herein, we propose and demonstrate an approach that yields the structure of protein-RNA complexes (RNP) solely from ssNMR data. Instead of using low-sensitivity magnetization transfer steps between heteronuclei of the protein and the RNA, we measure paramagnetic relaxation enhancement effects elicited on the RNA by a paramagnetic tag coupled to the protein. We demonstrate that this data, together with chemical-shift-perturbation data, yields an accurate structure of an RNP complex, starting from the bound structures of its components. The possibility of characterizing protein-RNA interactions by ssNMR may enable applications to large RNP complexes, whose structures are not accessible by other methods.Citation
Angew Chem Int Ed Engl. 2020 Feb 5. doi: 10.1002/anie.201915465.Affiliation
HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.Publisher
WileyPubMed ID
32023357Type
ArticleLanguage
enEISSN
1521-3773ae974a485f413a2113503eed53cd6c53
10.1002/anie.201915465
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