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dc.contributor.authorWittstein, K
dc.contributor.authorCordsmeier, A
dc.contributor.authorLambert, C
dc.contributor.authorWendt, L
dc.contributor.authorSir, E B
dc.contributor.authorWeber, J
dc.contributor.authorWurzler, N
dc.contributor.authorPetrini, L E
dc.contributor.authorStadler, M
dc.date.accessioned2020-03-17T15:38:54Z
dc.date.available2020-03-17T15:38:54Z
dc.date.issued2020-01-26
dc.identifier.citationStud Mycol. 2020 Jan 26;96:1-16. doi: 10.1016/j.simyco.2020.01.001. eCollection 2020 Jun.en_US
dc.identifier.issn0166-0616
dc.identifier.pmid32165986
dc.identifier.doi10.1016/j.simyco.2020.01.001
dc.identifier.urihttp://hdl.handle.net/10033/622211
dc.description.abstractRosellinia (Xylariaceae) is a large, cosmopolitan genus comprising over 130 species that have been defined based mainly on the morphology of their sexual morphs. The genus comprises both lignicolous and saprotrophic species that are frequently isolated as endophytes from healthy host plants, and important plant pathogens. In order to evaluate the utility of molecular phylogeny and secondary metabolite profiling to achieve a better basis for their classification, a set of strains was selected for a multi-locus phylogeny inferred from a combination of the sequences of the internal transcribed spacer region (ITS), the large subunit (LSU) of the nuclear rDNA, beta-tubulin (TUB2) and the second largest subunit of the RNA polymerase II (RPB2). Concurrently, various strains were surveyed for production of secondary metabolites. Metabolite profiling relied on methods with high performance liquid chromatography with diode array and mass spectrometric detection (HPLC-DAD/MS) as well as preparative isolation of the major components after re-fermentation followed by structure elucidation using nuclear magnetic resonance (NMR) spectroscopy and high resolution mass spectrometry (HR-MS). Two new and nine known isopimarane diterpenoids were identified during our mycochemical studies of two selected Dematophora strains and the metabolites were tested for biological activity. In addition, the nematicidal cyclodepsipeptide PF1022 A was purified and identified from a culture of Rosellinia corticium, which is the first time that this endophyte-derived drug precursor has been identified unambiguously from an ascospore-derived isolate of a Rosellinia species. While the results of this first HPLC profiling were largely inconclusive regarding the utility of secondary metabolites as genus-specific chemotaxonomic markers, the phylogeny clearly showed that species featuring a dematophora-like asexual morph were included in a well-defined clade, for which the genus Dematophora is resurrected. Dematophora now comprises all previously known important plant pathogens in the genus such as D. arcuata, D. bunodes, D. necatrix and D. pepo, while Rosellinia s. str. comprises those species that are known to have a geniculosporium-like or nodulisporium-like asexual morph, or where the asexual morph remains unknown. The extensive morphological studies of L.E. Petrini served as a basis to transfer several further species from Rosellinia to Dematophora, based on the morphology of their asexual morphs. However, most species of Rosellinia and allies still need to be recollected in fresh state, cultured, and studied for their morphology and their phylogenetic affinities before the infrageneric relationships can be clarified.en_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectDematophoraen_US
dc.subjectDematophora acutispora (Theiss.) C. Lambert, K. Wittstein & M. Stadleren_US
dc.subjectDematophora arcuata (Petch) C. Lambert, K. Wittstein & M. Stadleren_US
dc.subjectDematophora asperata (Massee ex Wakef.) Lambert, K. Wittstein & M. Stadleren_US
dc.subjectDematophora beccariana (Ces.) C. Lambert, K. Wittstein & M, Stadleren_US
dc.subjectDematophora boedijnii (L.E. Petrini) C. Lambert, K. Wittstein & M. Stadleren_US
dc.subjectDematophora bothrina (Berk. & Broome) C. Lambert, K. Wittstein & M. Stadleren_US
dc.subjectDematophora bunodes (Berk. & Broome) C. Lambert, K. Wittstein & M. Stadleren_US
dc.subjectDematophora buxi (Fabre) C. Lambert, K. Wittstein & M. Stadleren_US
dc.subjectDematophora compacta (Takemoto) C. Lambert, K. Wittstein & M. Stadleren_US
dc.subjectDematophora francisiae (L.E. Petrini) C. Lambert, K. Wittstein & M. Stadleren_US
dc.subjectDematophora freycinetiae (L.E. Petrini) C. Lambert, K. Wittstein & M. Stadleren_US
dc.subjectDematophora gigantea (Ellis & Everh.) C. Lambert, K. Wittstein & M. Stadleren_US
dc.subjectDematophora grantii (L.E. Petrini) C. Lambert, K. Wittstein & M. Stadleren_US
dc.subjectDematophora hsiehiae (L.E. Petrini) C. Lambert, K. Wittstein & M. Stadleren_US
dc.subjectDematophora hughesii (L.E. Petrini) C. Lambert, K. Wittstein & M. Stadleren_US
dc.subjectDematophora javaensis (L.E. Petrini) C. Lambert, K. Wittstein & M. Stadleren_US
dc.subjectDematophora macdonaldii (Bres.) C. Lambert, K. Wittstein & M. Stadleren_US
dc.subjectDematophora obregonii (L.E. Petrini) C. Lambert, K. Wittstein & M. Stadleren_US
dc.subjectDematophora obtusiostiolata (L.E. Petrini) C. Lambert, K. Wittstein & M. Stadleren_US
dc.subjectDematophora paraguayensis (Starbäck) C. Lambert, K. Wittstein & M. Stadleren_US
dc.subjectDematophora pepo (Pat.) C. Lambert, K. Wittstein & M. Stadleren_US
dc.subjectDematophora puiggarii (Pat.) C. Lambert, K. Wittstein & M. Stadleren_US
dc.subjectDematophora pyramidalis (Lar.N. Vassiljeva) C. Lambert, K. Wittstein & M. Stadleren_US
dc.subjectDematophora samuelsii (L.E. Petrini) C. Lambert, K. Wittstein & M. Stadleren_US
dc.subjectDematophora siggersii (L.E. Petrini) C. Lambert, K. Wittstein & M. Stadleren_US
dc.subjectGenus resurrectionen_US
dc.subjectIsopimarane diterpenoidsen_US
dc.subjectPF1022Aen_US
dc.subjectPolythetic taxonomyen_US
dc.subjectRoselliniaen_US
dc.subjectXylariaceaeen_US
dc.titleIdentification of species as producers of cyclodepsipeptide PF1022 A and resurrection of the genus as inferred from polythetic taxonomy.en_US
dc.typeArticleen_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalStudies in mycologyen_US
dc.source.volume96
dc.source.beginpage1
dc.source.endpage16
refterms.dateFOA2020-03-17T15:38:55Z
dc.source.journaltitleStudies in mycology
dc.source.countryNetherlands


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