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dc.contributor.authorGraziadei, Andrea
dc.contributor.authorGabel, Frank
dc.contributor.authorKirkpatrick, John
dc.contributor.authorCarlomagno, Teresa
dc.date.accessioned2020-04-07T09:49:31Z
dc.date.available2020-04-07T09:49:31Z
dc.date.issued2020-03-23
dc.identifier.citationElife. 2020 Mar 23;9. pii: 50027. doi: 10.7554/eLife.50027.en_US
dc.identifier.pmid32202498
dc.identifier.doi10.7554/eLife.50027
dc.identifier.urihttp://hdl.handle.net/10033/622223
dc.description.abstract2'-O-rRNA methylation, which is essential in eukaryotes and archaea, is catalysed by the Box C/D RNP complex in an RNA-guided manner. Despite the conservation of the methylation sites, the abundance of site-specific modifications shows variability across species and tissues, suggesting that rRNA methylation may provide a means of controlling gene expression. As all Box C/D RNPs are thought to adopt a similar structure, it remains unclear how the methylation efficiency is regulated. Here, we provide the first structural evidence that, in the context of the Box C/D RNP, the affinity of the catalytic module fibrillarin for the substrate-guide helix is dependent on the RNA sequence outside the methylation site, thus providing a mechanism by which both the substrate and guide RNA sequences determine the degree of methylation. To reach this result, we develop an iterative structure-calculation protocol that exploits the power of integrative structural biology to characterize conformational ensembles.en_US
dc.language.isoenen_US
dc.publishereLife Sciences Publicationsen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectBox C/D RNPen_US
dc.subjectNMRen_US
dc.subjectSASen_US
dc.subjectbiochemistryen_US
dc.subjectchemical biologyen_US
dc.subjectconformational equilibriumen_US
dc.subjectintegrative structural biologyen_US
dc.subjectmolecular biophysicsen_US
dc.subjectrRNA methylationen_US
dc.subjectstructural biologyen_US
dc.titleThe guide sRNA sequence determines the activity level of box C/D RNPs.en_US
dc.typeArticleen_US
dc.identifier.eissn2050-084X
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstraße 7, 38124 Braunschweig, Germany.en_US
dc.identifier.journaleLifeen_US
dc.source.volume9
refterms.dateFOA2020-04-07T09:49:31Z
dc.source.journaltitleeLife
dc.source.countryEngland


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