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dc.contributor.authorRoje, Blanka
dc.contributor.authorElek, Anamaria
dc.contributor.authorPalada, Vinko
dc.contributor.authorBom, Joana
dc.contributor.authorIljazović, Aida
dc.contributor.authorŠimić, Ana
dc.contributor.authorSušak, Lana
dc.contributor.authorVilović, Katarina
dc.contributor.authorStrowig, Till
dc.contributor.authorVlahoviček, Kristian
dc.contributor.authorTerzić, Janoš
dc.date.accessioned2020-04-07T13:26:13Z
dc.date.available2020-04-07T13:26:13Z
dc.date.issued2020-03-17
dc.identifier.citationMicroorganisms. 2020 Mar 17;8(3). pii: microorganisms8030421. doi: 10.3390/microorganisms8030421.en_US
dc.identifier.issn2076-2607
dc.identifier.pmid32192034
dc.identifier.doi10.3390/microorganisms8030421
dc.identifier.urihttp://hdl.handle.net/10033/622225
dc.description.abstractWe studied the effect of microbiota on the transcriptome and weight of the urinary bladder by comparing germ-free (GF) and specific pathogen-free (SPF) housed mice. In total, 97 genes were differently expressed (fold change > ±2; false discovery rate (FDR) p-value < 0.01) between the groups, including genes regulating circadian rhythm (Per1, Per2 and Per3), extracellular matrix (Spo1, Spon2), and neuromuscular synaptic transmission (Slc18a3, Slc5a7, Chrnb4, Chrna3, Snap25). The highest increase in expression was observed for immunoglobulin genes (Igkv1-122, Igkv4-68) of unknown function, but surprisingly the absence of microbiota did not change the expression of the genes responsible for recognizing microbes and their products. We found that urinary bladder weight was approximately 25% lighter in GF mice (p = 0.09 for males, p = 0.005 for females) and in mice treated with broad spectrum of antibiotics (p = 0.0002). In conclusion, our data indicate that microbiota is an important determinant of urinary bladder physiology controlling its gene expression and size.en_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectgerm-freeen_US
dc.subjectmicrobiomeen_US
dc.subjectmouseen_US
dc.subjecttranscriptomeen_US
dc.subjecturinary bladderen_US
dc.titleMicrobiota Alters Urinary Bladder Weight and Gene Expression.en_US
dc.typeArticleen_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstraße 7, 38124 Braunschweig, Germany.en_US
dc.identifier.journalMicroorganismsen_US
dc.source.volume8
dc.source.issue3
refterms.dateFOA2020-04-07T13:26:13Z
dc.source.journaltitleMicroorganisms
dc.source.countrySwitzerland


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