Capsule and fimbriae modulate the invasion of Haemophilus influenzae in a human blood-cerebrospinal fluid barrier model.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractThe Gram-negative bacterium Haemophilus influenzae (H. influenzae) can commensally colonize the upper respiratory tract, but also cause life threatening disease including epiglottitis, sepsis and meningitis. The H. influenzae capsule protects the bacteria against both phagocytosis and opsonization. Encapsulated H. influenzae strains are classified into serotypes ranging from a to f dependent on their distinct polysaccharide capsule. Due to the implementation of vaccination the incidence of invasive H. influenzae type b (Hib) infections has strongly decreased and infections with other capsulated types, including H. influenzae type f (Hif), are emerging. The pathogenesis of H. influenzae meningitis is not clarified. To enter the central nervous system (CNS) the bacteria generally have to cross either the blood-brain barrier (BBB) or the blood-cerebrospinal fluid barrier (BSCFB). Using a cell culture model of the BCSFB based on human choroid plexus papilloma (HIBCPP) cells and different H. influenzae strains we investigated whether Hib and Hif invade the cells, and if invasion differs between encapsulated vs. capsular-deficient and fimbriated vs. non-fimbriated variants. We find that Hib can adhere to and invade into HIBCPP cells. Invasion occurs in a strongly polar fashion, since the bacteria enter the cells preferentially from the basolateral "blood "side. Fimbriae and capsule attenuate invasion into choroid plexus (CP) epithelial cells, and capsulation can influence the bacterial distribution pattern. Finally, analysis of clinical Hib and Hif isolates confirms the detected invasive properties of H. influenzae. Our data point to roles of capsule and fimbriae during invasion of CP epithelial cells.
CitationInt J Med Microbiol. 2018 Oct;308(7):829-839. doi: 10.1016/j.ijmm.2018.07.004. Epub 2018 Jul 17.
AffiliationHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International
- Non-Typeable <i>Haemophilus influenzae</i> Invade Choroid Plexus Epithelial Cells in a Polar Fashion.
- Authors: Wegele C, Stump-Guthier C, Moroniak S, Weiss C, Rohde M, Ishikawa H, Schroten H, Schwerk C, Karremann M, Borkowski J
- Issue date: 2020 Aug 10
- Haemophilus influenzae type-b and non-b-type invasive diseases in urban children (<5years) of Bangladesh: implications for therapy and vaccination.
- Authors: Rahman M, Hossain S, Baqui AH, Shoma S, Rashid H, Nahar N, Zaman MK, Khatun F
- Issue date: 2008 Mar
- Haemophilus influenzae serotype a as a cause of serious invasive infections.
- Authors: Ulanova M, Tsang RSW
- Issue date: 2014 Jan
- Comparative genomic analysis reveals distinct genotypic features of the emerging pathogen Haemophilus influenzae type f.
- Authors: Su YC, Resman F, Hörhold F, Riesbeck K
- Issue date: 2014 Jan 18
- Analysis of non-typeable Haemophilus influenzae in invasive disease reveals lack of the capsule locus.
- Authors: Lâm TT, Claus H, Frosch M, Vogel U
- Issue date: 2016 Jan