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dc.contributor.authorGuo, Xiaojing
dc.contributor.authorLuo, Siwei
dc.contributor.authorAmidani, Davide
dc.contributor.authorRivetti, Claudio
dc.contributor.authorPieraccini, Giuseppe
dc.contributor.authorPioselli, Barbara
dc.contributor.authorCatinella, Silvia
dc.contributor.authorMurgia, Xabi
dc.contributor.authorSalomone, Fabrizio
dc.contributor.authorXu, Yaling
dc.contributor.authorDong, Ying
dc.contributor.authorSun, Bo
dc.date.accessioned2020-04-15T15:06:55Z
dc.date.available2020-04-15T15:06:55Z
dc.date.issued2020-03-13
dc.identifier.citationPLoS One. 2020 Mar 13;15(3):e0230229. doi: 10.1371/journal.pone.0230229. eCollection 2020.en_US
dc.identifier.pmid32168331
dc.identifier.doi10.1371/journal.pone.0230229
dc.identifier.urihttp://hdl.handle.net/10033/622232
dc.description.abstractPoractant alfa and Calsurf are two natural surfactants widely used in China for the treatment of neonatal respiratory distress syndrome, which are extracted from porcine and calf lungs, respectively. The purpose of this experimental study was to compare their in vitro characteristics and in vivo effects in the improvement of pulmonary function and protection of lung injury. The biophysical properties, ultrastructure, and lipid composition of both surfactant preparations were respectively analysed in vitro by means of Langmuir-Blodgett trough (LBT), atomic force microscopy (AFM), and liquid-chromatography mass-spectrometry (LC-MS). Then, as core pharmacological activity, both head-to-head (100 and 200 mg/kg for both surfactants) and licensed dose comparisons (70 mg/kg Calsurf vs. 200 mg/kg Poractant alfa) between the two surfactants were conducted as prophylaxis in preterm rabbits with primary surfactant deficiency, assessing survival time and rate and dynamic compliance of the respiratory system (Cdyn). Intrapulmonary surfactant pools, morphometric volume density as alveolar expansion (Vv), and lung injury scores were determined post mortem. AFM and LC-MS analysis revealed qualitative differences in the ultrastructure as well as in the lipid composition of both preparations. Calsurf showed a longer plateau region of the LBT isotherm and lower film compressibility. In vivo, both surfactant preparations improved Cdyn at any dose, although maximum benefits in terms of Vv and intrapulmonary surfactant pools were seen with the 200 mg/kg dose in both surfactants. The group of animals treated with 200 mg/kg of Poractant alfa showed a prolonged survival time and rate compared to untreated but ventilated controls, and significantly ameliorated lung injury compared to Calsurf at any dose, including 200 mg/kg. The overall outcomes suggest the pulmonary effects to be dose dependent for both preparations. The group of animals treated with 200 mg/kg of Poractant alfa showed a significant reduction of mortality. Compared to Calsurf, Poractant alfa exerted better effects if licensed doses were compared, which requires further investigation.en_US
dc.language.isoenen_US
dc.publisherPLOSen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleIn vitro characterization and in vivo comparison of the pulmonary outcomes of Poractant alfa and Calsurf in ventilated preterm rabbits.en_US
dc.typeArticleen_US
dc.identifier.eissn1932-6203
dc.contributor.departmentHIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1 66123 Saarbrücken, Germany.en_US
dc.identifier.journalPloS oneen_US
dc.source.volume15
dc.source.issue3
dc.source.beginpagee0230229
dc.source.endpage
refterms.dateFOA2020-04-15T15:06:56Z
dc.source.journaltitlePloS one
dc.source.countryUnited States


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