Grad-seq in a Gram-positive bacterium reveals exonucleolytic sRNA activation in competence control.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Di Giorgio, Silvia
Vanselow, Jens T
Förstner, Konrad U
MetadataShow full item record
AbstractRNA-protein interactions are the crucial basis for many steps of bacterial gene expression, including post-transcriptional control by small regulatory RNAs (sRNAs). In stark contrast to recent progress in the analysis of Gram-negative bacteria, knowledge about RNA-protein complexes in Gram-positive species remains scarce. Here, we used the Grad-seq approach to draft a comprehensive landscape of such complexes in Streptococcus pneumoniae, in total determining the sedimentation profiles of ~ 88% of the transcripts and ~ 62% of the proteins of this important human pathogen. Analysis of in-gradient distributions and subsequent tag-based protein capture identified interactions of the exoribonuclease Cbf1/YhaM with sRNAs that control bacterial competence for DNA uptake. Unexpectedly, the nucleolytic activity of Cbf1 stabilizes these sRNAs, thereby promoting their function as repressors of competence. Overall, these results provide the first RNA/protein complexome resource of a Gram-positive species and illustrate how this can be utilized to identify new molecular factors with functions in RNA-based regulation of virulence-relevant pathways.
CitationEMBO J. 2020 Mar 30:e103852. doi: 10.15252/embj.2019103852.
AffiliationHIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Shneider Strasse 2, 97080 Würzburg, Germany.
JournalThe EMBO journal
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International
- Redefining the Small Regulatory RNA Transcriptome in Streptococcus pneumoniae Serotype 2 Strain D39.
- Authors: Sinha D, Zimmer K, Cameron TA, Rusch DB, Winkler ME, De Lay NR
- Issue date: 2019 Jul 15
- Analysis of the RNA and Protein Complexome by Grad-seq.
- Authors: Hör J, Vogel J
- Issue date: 2021
- Control of virulence by small RNAs in Streptococcus pneumoniae.
- Authors: Mann B, van Opijnen T, Wang J, Obert C, Wang YD, Carter R, McGoldrick DJ, Ridout G, Camilli A, Tuomanen EI, Rosch JW
- Issue date: 2012
- A Grad-seq View of RNA and Protein Complexes in Pseudomonas aeruginosa under Standard and Bacteriophage Predation Conditions.
- Authors: Gerovac M, Wicke L, Chihara K, Schneider C, Lavigne R, Vogel J
- Issue date: 2021 Feb 9
- Small regulatory RNAs from low-GC Gram-positive bacteria.
- Authors: Brantl S, Brückner R
- Issue date: 2014