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dc.contributor.authorKouakanou, Léonce
dc.contributor.authorPeters, Christian
dc.contributor.authorSun, Qiwei
dc.contributor.authorFloess, Stefan
dc.contributor.authorBhat, Jaydeep
dc.contributor.authorHuehn, Jochen
dc.contributor.authorKabelitz, Dieter
dc.date.accessioned2020-05-12T09:06:17Z
dc.date.available2020-05-12T09:06:17Z
dc.date.issued2020-04-16
dc.identifier.citationSci Rep. 2020 Apr 16;10(1):6550. doi: 10.1038/s41598-020-63572-w.en_US
dc.identifier.pmid32300237
dc.identifier.doi10.1038/s41598-020-63572-w
dc.identifier.urihttp://hdl.handle.net/10033/622256
dc.description.abstractHuman γδ T cells are potent cytotoxic effector cells, produce a variety of cytokines, and can acquire regulatory activity. Induction of FOXP3, the key transcription factor of regulatory T cells (Treg), by TGF-β in human Vγ9 Vδ2 T cells has been previously reported. Vitamin C is an antioxidant and acts as multiplier of DNA hydroxymethylation. Here we have investigated the effect of the more stable phospho-modified Vitamin C (pVC) on TGF-β-induced FOXP3 expression and the resulting regulatory activity of highly purified human Vγ9 Vδ2 T cells. pVC significantly increased the TGF-β-induced FOXP3 expression and stability and also increased the suppressive activity of Vγ9 Vδ2 T cells. Importantly, pVC induced hypomethylation of the Treg-specific demethylated region (TSDR) in the FOXP3 gene. Genome-wide methylation analysis by Reduced Representation Bisulfite Sequencing additionally revealed differentially methylated regions in several important genes upon pVC treatment of γδ T cells. While Vitamin C also enhances effector functions of Vγ9 Vδ2 T cells in the absence of TGF-β, our results demonstrate that pVC potently increases the suppressive activity and FOXP3 expression in TGF-β-treated Vγ9 Vδ2 T cells by epigenetic modification of the FOXP3 geneen_US
dc.language.isoenen_US
dc.publisherNature Publishing Groupen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleVitamin C supports conversion of human γδ T cells into FOXP3-expressing regulatory cells by epigenetic regulation.en_US
dc.typeArticleen_US
dc.identifier.eissn2045-2322
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalScientific reportsen_US
dc.source.volume10
dc.source.issue1
dc.source.beginpage6550
dc.source.endpage
refterms.dateFOA2020-05-12T09:06:18Z
dc.source.journaltitleScientific reports
dc.source.countryEngland


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