Vitamin C supports conversion of human γδ T cells into FOXP3-expressing regulatory cells by epigenetic regulation.
dc.contributor.author | Kouakanou, Léonce | |
dc.contributor.author | Peters, Christian | |
dc.contributor.author | Sun, Qiwei | |
dc.contributor.author | Floess, Stefan | |
dc.contributor.author | Bhat, Jaydeep | |
dc.contributor.author | Huehn, Jochen | |
dc.contributor.author | Kabelitz, Dieter | |
dc.date.accessioned | 2020-05-12T09:06:17Z | |
dc.date.available | 2020-05-12T09:06:17Z | |
dc.date.issued | 2020-04-16 | |
dc.identifier.citation | Sci Rep. 2020 Apr 16;10(1):6550. doi: 10.1038/s41598-020-63572-w. | en_US |
dc.identifier.pmid | 32300237 | |
dc.identifier.doi | 10.1038/s41598-020-63572-w | |
dc.identifier.uri | http://hdl.handle.net/10033/622256 | |
dc.description.abstract | Human γδ T cells are potent cytotoxic effector cells, produce a variety of cytokines, and can acquire regulatory activity. Induction of FOXP3, the key transcription factor of regulatory T cells (Treg), by TGF-β in human Vγ9 Vδ2 T cells has been previously reported. Vitamin C is an antioxidant and acts as multiplier of DNA hydroxymethylation. Here we have investigated the effect of the more stable phospho-modified Vitamin C (pVC) on TGF-β-induced FOXP3 expression and the resulting regulatory activity of highly purified human Vγ9 Vδ2 T cells. pVC significantly increased the TGF-β-induced FOXP3 expression and stability and also increased the suppressive activity of Vγ9 Vδ2 T cells. Importantly, pVC induced hypomethylation of the Treg-specific demethylated region (TSDR) in the FOXP3 gene. Genome-wide methylation analysis by Reduced Representation Bisulfite Sequencing additionally revealed differentially methylated regions in several important genes upon pVC treatment of γδ T cells. While Vitamin C also enhances effector functions of Vγ9 Vδ2 T cells in the absence of TGF-β, our results demonstrate that pVC potently increases the suppressive activity and FOXP3 expression in TGF-β-treated Vγ9 Vδ2 T cells by epigenetic modification of the FOXP3 gene | en_US |
dc.language.iso | en | en_US |
dc.publisher | Nature Publishing Group | en_US |
dc.rights | Attribution-NonCommercial-ShareAlike 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | * |
dc.title | Vitamin C supports conversion of human γδ T cells into FOXP3-expressing regulatory cells by epigenetic regulation. | en_US |
dc.type | Article | en_US |
dc.identifier.eissn | 2045-2322 | |
dc.contributor.department | HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany. | en_US |
dc.identifier.journal | Scientific reports | en_US |
dc.source.volume | 10 | |
dc.source.issue | 1 | |
dc.source.beginpage | 6550 | |
dc.source.endpage | ||
refterms.dateFOA | 2020-05-12T09:06:18Z | |
dc.source.journaltitle | Scientific reports | |
dc.source.country | England |