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dc.contributor.authorCui, Shuna
dc.contributor.authorLi, Minghui
dc.contributor.authorHassan, Rabeay Y A
dc.contributor.authorHeintz-Buschart, Anna
dc.contributor.authorWang, Junsong
dc.contributor.authorBilitewski, Ursula
dc.date.accessioned2020-05-13T13:32:11Z
dc.date.available2020-05-13T13:32:11Z
dc.date.issued2020-04-15
dc.identifier.citationmSphere. 2020 Apr 15;5(2). pii: 5/2/e00016-20. doi: 10.1128/mSphere.00016-20.en_US
dc.identifier.pmid32295866
dc.identifier.doi10.1128/mSphere.00016-20
dc.identifier.urihttp://hdl.handle.net/10033/622260
dc.description.abstractCandida albicans adapts to various conditions in different body niches by regulating gene expression, protein synthesis, and metabolic pathways. These adaptive reactions not only allow survival but also influence the interaction with host cells, which is governed by the composition and structure of the fungal cell wall. Numerous studies had shown linkages between mitochondrial functionality, cell wall integrity and structure, and pathogenicity. Thus, we decided to inhibit single complexes of the respiratory chain of C. albicans and to analyze the resultant interaction with macrophages via their phagocytic activity. Remarkably, inhibition of the fungal bc1 complex by antimycin A increased phagocytosis, which correlated with an increased accessibility of β-glucans. To contribute to mechanistic insights, we performed metabolic studies, which highlighted significant changes in the abundance of constituents of the plasma membrane. Collectively, our results reinforce the strong linkage between fungal energy metabolism and other components of fungal physiology, which also determine the vulnerability to immune defense reactions.IMPORTANCE The yeast Candida albicans is one of the major fungal human pathogens, for which new therapeutic approaches are required. We aimed at enhancements of the phagocytosis efficacy of macrophages by targeting the cell wall structure of C. albicans, as the coverage of the β-glucan layer by mannans is one of the immune escape mechanisms of the fungus. We unambiguously show that inhibition of the fungal bc1 complex correlates with increased accessibilities of β-glucans and improved phagocytosis efficiency. Metabolic studies proved not only the known direct effects on reactive oxygen species (ROS) production and fermentative pathways but also the clear downregulation of the ergosterol pathway and upregulation of unsaturated fatty acids. The changed composition of the plasma membrane could also influence the interaction with the overlying cell wall. Thus, our work highlights the far-reaching relevance of energy metabolism, indirectly also for host-pathogen interactions, without affecting viability.en_US
dc.language.isoenen_US
dc.publisherAmerican Society of Microbiologyen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectantimycin Aen_US
dc.subjectcomplex IIIen_US
dc.subjectmetabolismen_US
dc.subjectphagocytosisen_US
dc.subjectrespiratory chainen_US
dc.titleInhibition of Respiration of Candida albicans by Small Molecules Increases Phagocytosis Efficacy by Macrophages.en_US
dc.typeArticleen_US
dc.identifier.eissn2379-5042
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstraße 7, 38124 Braunschweig, Germany.en_US
dc.identifier.journalmSphereen_US
dc.source.volume5
dc.source.issue2
refterms.dateFOA2020-05-13T13:32:12Z
dc.source.journaltitlemSphere
dc.source.countryUnited States


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