Tunable self-cleaving ribozymes for modulating gene expression in eukaryotic systems.
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Authors
Jacobsen, ThomasYi, Gloria
Al Asafen, Hadel
Jermusyk, Ashley A
Beisel, Chase L
Reeves, Gregory T
Issue Date
2020-04-30
Metadata
Show full item recordAbstract
Advancements in the field of synthetic biology have been possible due to the development of genetic tools that are able to regulate gene expression. However, the current toolbox of gene regulatory tools for eukaryotic systems have been outpaced by those developed for simple, single-celled systems. Here, we engineered a set of gene regulatory tools by combining self-cleaving ribozymes with various upstream competing sequences that were designed to disrupt ribozyme self-cleavage. As a proof-of-concept, we were able to modulate GFP expression in mammalian cells, and then showed the feasibility of these tools in Drosophila embryos. For each system, the fold-reduction of gene expression was influenced by the location of the self-cleaving ribozyme/upstream competing sequence (i.e. 5' vs. 3' untranslated region) and the competing sequence used. Together, this work provides a set of genetic tools that can be used to tune gene expression across various eukaryotic systems.Citation
PLoS One. 2020;15(4):e0232046. Published 2020 Apr 30. doi:10.1371/journal.pone.0232046Affiliation
HIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Shneider Strasse 2, 97080 Würzburg, Germany.Publisher
PLOSJournal
PloS onePubMed ID
32352996Type
ArticleLanguage
enEISSN
1932-6203ae974a485f413a2113503eed53cd6c53
10.1371/journal.pone.0232046
Scopus Count
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- Creative Commons
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