Increasing storage stability of freeze-dried plasma using trehalose.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Wolkers, Willem F
MetadataShow full item record
AbstractPreservation of blood plasma in the dried state would facilitate long-term storage and transport at ambient temperatures, without the need of to use liquid nitrogen tanks or freezers. The aim of this study was to investigate the feasibility of dry preservation of human plasma, using sugars as lyoprotectants, and evaluate macromolecular stability of plasma components during storage. Blood plasma from healthy donors was freeze dried using 0-10% glucose, sucrose, or trehalose, and stored at various temperatures. Differential scanning calorimetry was used to measure the glass transition temperatures of freeze-dried samples. Protein aggregation, the overall protein secondary structure, and oxidative damage were studied under different storage conditions. Differential scanning calorimetry measurements showed that plasma freeze-dried with glucose, sucrose and trehalose have glass transition temperatures of respectively 72±3.4°C, 46±11°C, 15±2.4°C. It was found that sugars diminish freeze-drying induced protein aggregation in a dose-dependent manner, and that a 10% (w/v) sugar concentration almost entirely prevents protein aggregation. Protein aggregation after rehydration coincided with relatively high contents of β-sheet structures in the dried state. Trehalose reduced the rate of protein aggregation during storage at elevated temperatures, and plasma that is freeze- dried plasma with trehalose showed a reduced accumulation of reactive oxygen species and protein oxidation products during storage. In conclusion, freeze-drying plasma with trehalose provides an attractive alternative to traditional cryogenic preservation
CitationPLoS One. 2020 Jun 11;15(6):e0234502. doi: 10.1371/journal.pone.0234502 eCollection 2020.
AffiliationHIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Shneider Strasse 2, 97080 Würzburg, Germany.
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International
- Freeze-drying of mammalian cells using trehalose: preservation of DNA integrity.
- Authors: Zhang M, Oldenhof H, Sydykov B, Bigalk J, Sieme H, Wolkers WF
- Issue date: 2017 Jul 24
- Trehalose and hyaluronic acid coordinately stabilized freeze-dried pancreatic kininogenase.
- Authors: Zhang Y, Ji B, Ling P, Zhang T
- Issue date: 2007 Jan
- Distinct effects of sucrose and trehalose on protein stability during supercritical fluid drying and freeze-drying.
- Authors: Jovanović N, Bouchard A, Hofland GW, Witkamp GJ, Crommelin DJ, Jiskoot W
- Issue date: 2006 Mar
- Isothermal vitrification methodology development for non-cryogenic storage of archival human sera.
- Authors: Less R, Boylan KL, Skubitz AP, Aksan A
- Issue date: 2013 Apr
- Optimization of storage stability of lyophilized actin using combinations of disaccharides and dextran.
- Authors: Allison SD, Manning MC, Randolph TW, Middleton K, Davis A, Carpenter JF
- Issue date: 2000 Feb