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dc.contributor.authorBrogna, Raffaele
dc.contributor.authorOldenhof, Harriëtte
dc.contributor.authorSieme, Harald
dc.contributor.authorFigueiredo, Constança
dc.contributor.authorKerrinnes, Tobias
dc.contributor.authorWolkers, Willem F
dc.date.accessioned2020-06-26T14:16:22Z
dc.date.available2020-06-26T14:16:22Z
dc.date.issued2020-06-11
dc.identifier.citationPLoS One. 2020 Jun 11;15(6):e0234502. doi: 10.1371/journal.pone.0234502 eCollection 2020.en_US
dc.identifier.pmid32525915
dc.identifier.doi10.1371/journal.pone.0234502
dc.identifier.urihttp://hdl.handle.net/10033/622316
dc.description.abstractPreservation of blood plasma in the dried state would facilitate long-term storage and transport at ambient temperatures, without the need of to use liquid nitrogen tanks or freezers. The aim of this study was to investigate the feasibility of dry preservation of human plasma, using sugars as lyoprotectants, and evaluate macromolecular stability of plasma components during storage. Blood plasma from healthy donors was freeze dried using 0-10% glucose, sucrose, or trehalose, and stored at various temperatures. Differential scanning calorimetry was used to measure the glass transition temperatures of freeze-dried samples. Protein aggregation, the overall protein secondary structure, and oxidative damage were studied under different storage conditions. Differential scanning calorimetry measurements showed that plasma freeze-dried with glucose, sucrose and trehalose have glass transition temperatures of respectively 72±3.4°C, 46±11°C, 15±2.4°C. It was found that sugars diminish freeze-drying induced protein aggregation in a dose-dependent manner, and that a 10% (w/v) sugar concentration almost entirely prevents protein aggregation. Protein aggregation after rehydration coincided with relatively high contents of β-sheet structures in the dried state. Trehalose reduced the rate of protein aggregation during storage at elevated temperatures, and plasma that is freeze- dried plasma with trehalose showed a reduced accumulation of reactive oxygen species and protein oxidation products during storage. In conclusion, freeze-drying plasma with trehalose provides an attractive alternative to traditional cryogenic preservationen_US
dc.language.isoenen_US
dc.publisherPLOSen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleIncreasing storage stability of freeze-dried plasma using trehalose.en_US
dc.typeArticleen_US
dc.identifier.eissn1932-6203
dc.contributor.departmentHIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Shneider Strasse 2, 97080 Würzburg, Germany.en_US
dc.identifier.journalPloS oneen_US
dc.source.volume15
dc.source.issue6
dc.source.beginpagee0234502
dc.source.endpage
refterms.dateFOA2020-06-26T14:16:23Z
dc.source.journaltitlePloS one
dc.source.countryUnited States


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Attribution-NonCommercial-ShareAlike 4.0 International
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