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dc.contributor.authorDeng, Zhi-Luo
dc.contributor.authorSztajer, Helena
dc.contributor.authorJarek, Michael
dc.contributor.authorBhuju, Sabin
dc.contributor.authorWagner-Döbler, Irene
dc.date.accessioned2020-07-07T12:47:27Z
dc.date.available2020-07-07T12:47:27Z
dc.date.issued2018-02-06
dc.identifier.citationFront Microbiol. 2018;9:124. Published 2018 Feb 6.en_US
dc.identifier.issn1664-302X
dc.identifier.pmid29467738
dc.identifier.doi10.3389/fmicb.2018.00124
dc.identifier.urihttp://hdl.handle.net/10033/622344
dc.description.abstractPeriodontitis is a worldwide prevalent oral disease which results from dysbiosis of the periodontal microbiome. Some of the most active microbial players, e.g., Porphyromonas gingivalis, Treponema denticola, and Fusobacterium nucleatum, have extensively been studied in the laboratory, but it is unclear to which extend these findings can be transferred to in vivo conditions. Here we show that the transcriptional profiles of P. gingivalis, T. denticola, and F. nucleatum in the periodontal niche are distinct from those in single laboratory culture and exhibit functional similarities. GO (gene ontology) term enrichment analysis showed up-regulation of transporters, pathogenicity related traits and hemin/heme uptake mechanisms for all three species in vivo. Differential gene expression analysis revealed that cysteine proteases, transporters and hemin/heme-binding proteins were highly up-regulated in the periodontal niche, while genes involved in DNA modification were down-regulated. The data suggest strong interactions between those three species regarding protein degradation, iron up-take, and mobility in vivo, explaining their enhanced synergistic pathogenicity. We discovered a strikingly high frequency of Single Nucleotide Polymorphisms (SNPs) in vivo. For F. nucleatum we discovered a total of 127,729 SNPs in periodontal niche transcripts, which were found in similar frequency in health and disease and covered the entire genome, suggesting continuous evolution in the host. We conclude that metabolic interactions shape gene expression in vivo. Great caution is required when inferring pathogenicity of microbes from laboratory data, and microdiversity is an important adaptive trait of natural communities.en_US
dc.language.isoenen_US
dc.publisherFrontiersen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectFusobacterium nucleatumen_US
dc.subjectPorphyromonas gingivalisen_US
dc.subjectSNPsen_US
dc.subjectTreponema denticolaen_US
dc.subjectinter-species interactionen_US
dc.subjectmetatranscriptomeen_US
dc.subjectperiodontitisen_US
dc.titleWorlds Apart - Transcriptome Profiles of Key Oral Microbes in the Periodontal Pocket Compared to Single Laboratory Culture Reflect Synergistic Interactions.en_US
dc.typeArticleen_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalFrontiers in microbiologyen_US
dc.source.volume9
dc.source.beginpage124
dc.source.endpage
refterms.dateFOA2020-07-07T12:47:28Z
dc.source.journaltitleFrontiers in microbiology
dc.source.countrySwitzerland


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