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dc.contributor.authorMika-Gospodorz, Bozena
dc.contributor.authorGiengkam, Suparat
dc.contributor.authorWestermann, Alexander J
dc.contributor.authorWongsantichon, Jantana
dc.contributor.authorKion-Crosby, Willow
dc.contributor.authorChuenklin, Suthida
dc.contributor.authorWang, Loo Chien
dc.contributor.authorSunyakumthorn, Piyanate
dc.contributor.authorSobota, Radoslaw M
dc.contributor.authorSubbian, Selvakumar
dc.contributor.authorVogel, Jörg
dc.contributor.authorBarquist, Lars
dc.contributor.authorSalje, Jeanne
dc.date.accessioned2020-07-22T11:25:56Z
dc.date.available2020-07-22T11:25:56Z
dc.date.issued2020-07-03
dc.identifier.citationNat Commun. 2020;11(1):3363. Published 2020 Jul 3. doi:10.1038/s41467-020-17094-8.en_US
dc.identifier.pmid32620750
dc.identifier.doi10.1038/s41467-020-17094-8
dc.identifier.urihttp://hdl.handle.net/10033/622355
dc.description.abstractStudying emerging or neglected pathogens is often challenging due to insufficient information and absence of genetic tools. Dual RNA-seq provides insights into host-pathogen interactions, and is particularly informative for intracellular organisms. Here we apply dual RNA-seq to Orientia tsutsugamushi (Ot), an obligate intracellular bacterium that causes the vector-borne human disease scrub typhus. Half the Ot genome is composed of repetitive DNA, and there is minimal collinearity in gene order between strains. Integrating RNA-seq, comparative genomics, proteomics, and machine learning to study the transcriptional architecture of Ot, we find evidence for wide-spread post-transcriptional antisense regulation. Comparing the host response to two clinical isolates, we identify distinct immune response networks for each strain, leading to predictions of relative virulence that are validated in a mouse infection model. Thus, dual RNA-seq can provide insight into the biology and host-pathogen interactions of a poorly characterized and genetically intractable organism such as Ot.en_US
dc.language.isoenen_US
dc.publisherNature Publishing Groupen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleDual RNA-seq of Orientia tsutsugamushi informs on host-pathogen interactions for this neglected intracellular human pathogen.en_US
dc.typeArticleen_US
dc.identifier.eissn2041-1723
dc.contributor.departmentHIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Shneider Strasse 2, 97080 Würzburg, Germany.en_US
dc.identifier.journalNature communicationsen_US
dc.source.volume11
dc.source.issue1
dc.source.beginpage3363
dc.source.endpage
refterms.dateFOA2020-07-22T11:25:58Z
dc.source.journaltitleNature communications
dc.source.countryEngland


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