Dual RNA-seq of Orientia tsutsugamushi informs on host-pathogen interactions for this neglected intracellular human pathogen.
dc.contributor.author | Mika-Gospodorz, Bozena | |
dc.contributor.author | Giengkam, Suparat | |
dc.contributor.author | Westermann, Alexander J | |
dc.contributor.author | Wongsantichon, Jantana | |
dc.contributor.author | Kion-Crosby, Willow | |
dc.contributor.author | Chuenklin, Suthida | |
dc.contributor.author | Wang, Loo Chien | |
dc.contributor.author | Sunyakumthorn, Piyanate | |
dc.contributor.author | Sobota, Radoslaw M | |
dc.contributor.author | Subbian, Selvakumar | |
dc.contributor.author | Vogel, Jörg | |
dc.contributor.author | Barquist, Lars | |
dc.contributor.author | Salje, Jeanne | |
dc.date.accessioned | 2020-07-22T11:25:56Z | |
dc.date.available | 2020-07-22T11:25:56Z | |
dc.date.issued | 2020-07-03 | |
dc.identifier.citation | Nat Commun. 2020;11(1):3363. Published 2020 Jul 3. doi:10.1038/s41467-020-17094-8. | en_US |
dc.identifier.pmid | 32620750 | |
dc.identifier.doi | 10.1038/s41467-020-17094-8 | |
dc.identifier.uri | http://hdl.handle.net/10033/622355 | |
dc.description.abstract | Studying emerging or neglected pathogens is often challenging due to insufficient information and absence of genetic tools. Dual RNA-seq provides insights into host-pathogen interactions, and is particularly informative for intracellular organisms. Here we apply dual RNA-seq to Orientia tsutsugamushi (Ot), an obligate intracellular bacterium that causes the vector-borne human disease scrub typhus. Half the Ot genome is composed of repetitive DNA, and there is minimal collinearity in gene order between strains. Integrating RNA-seq, comparative genomics, proteomics, and machine learning to study the transcriptional architecture of Ot, we find evidence for wide-spread post-transcriptional antisense regulation. Comparing the host response to two clinical isolates, we identify distinct immune response networks for each strain, leading to predictions of relative virulence that are validated in a mouse infection model. Thus, dual RNA-seq can provide insight into the biology and host-pathogen interactions of a poorly characterized and genetically intractable organism such as Ot. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Nature Publishing Group | en_US |
dc.rights | Attribution-NonCommercial-ShareAlike 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | * |
dc.title | Dual RNA-seq of Orientia tsutsugamushi informs on host-pathogen interactions for this neglected intracellular human pathogen. | en_US |
dc.type | Article | en_US |
dc.identifier.eissn | 2041-1723 | |
dc.contributor.department | HIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Shneider Strasse 2, 97080 Würzburg, Germany. | en_US |
dc.identifier.journal | Nature communications | en_US |
dc.source.volume | 11 | |
dc.source.issue | 1 | |
dc.source.beginpage | 3363 | |
dc.source.endpage | ||
refterms.dateFOA | 2020-07-22T11:25:58Z | |
dc.source.journaltitle | Nature communications | |
dc.source.country | England |