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dc.contributor.authorBolten, Svenja Nicolin
dc.contributor.authorKnoll, Anne-Sophie
dc.contributor.authorLi, Zhaopeng
dc.contributor.authorGellermann, Pia
dc.contributor.authorPepelanova, Iliyana
dc.contributor.authorRinas, Ursula
dc.contributor.authorScheper, Thomas
dc.date.accessioned2020-07-23T08:18:25Z
dc.date.available2020-07-23T08:18:25Z
dc.date.issued2020-08
dc.identifier.issn0021-9673
dc.identifier.doi10.1016/j.chroma.2020.461367
dc.identifier.urihttp://hdl.handle.net/10033/622357
dc.description.abstractThis paper analyzes the use of animal-component free chromatographic materials for the efficient purifi- cation of the human fibroblast growth factor 2 (hFGF-2). hFGF-2 is produced in Escherichia coli and pu- rified via three different chromatography steps, which include a strong cation exchange chromatography as a capture step, followed by heparin affinity chromatography and an anion exchange chromatography as a polishing step. The affinity chromatography step is based on the animal-derived material heparin. Chemically produced ligands provide a viable alternative to animal-derived components in production processes, since they are characterized by a defined structure which leads to reproducible results and a broad range of applications. The alternative ligands can be assigned to adsorber of the mixed-mode chromatography (MMC) and pseudo-affinity chromatography. Eight different animal-component free materials used as adsorbers in MMC or pseudo-affinity chromatog- raphy were tested as a substitute for heparin. The MMCs were cation exchangers characterized with fur- ther functional residues. The ligands of the pseudo-affinity chromatography were heparin-like ligands which are based on heparin’s molecular structure. The alternative methods were tested as a capture step and in combination with another chromatographic step in the purification procedure of hFGF-2. In each downstream step purity, recovery and yield were analysed and compared to the conventional downstream process. Two types of MMC –the column Foresight TM Nuvia TM cPrime TM from Bio-Rad Laboratories and the col- umn HiTrap TM Capto TM MMC from GE Healthcare Life Sciences - can be regarded as effective animal- component free alternatives to the heparin - based adsorber.en_US
dc.description.sponsorshipGraded Implants FOR2180en_US
dc.language.isoenen_US
dc.publisherElsevier BVen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttps://www.elsevier.com/tdm/userlicense/1.0/
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectOrganic Chemistryen_US
dc.subjectAnalytical Chemistryen_US
dc.subjectBiochemistryen_US
dc.subjectGeneral Medicineen_US
dc.titlePurification of the human fibroblast growth factor 2 using novel animal-component free materialsen_US
dc.typeArticleen_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalJournal of Chromatography Aen_US
dc.identifier.piiS0021967320306440
dc.source.volume1626
dc.source.beginpage461367
refterms.dateFOA2020-07-23T08:18:26Z
dc.source.journaltitleJournal of Chromatography A


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