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dc.contributor.authorArmando, Federico
dc.contributor.authorGambini, Matteo
dc.contributor.authorCorradi, Attilio
dc.contributor.authorBecker, Kathrin
dc.contributor.authorMarek, Katarzyna
dc.contributor.authorPfankuche, Vanessa Maria
dc.contributor.authorMergani, Ahmed Elmonastir
dc.contributor.authorBrogden, Graham
dc.contributor.authorde Buhr, Nicole
dc.contributor.authorVon Köckritz-Blickwede, Maren
dc.contributor.authorNaim, Hassan Y
dc.contributor.authorBaumgärtner, Wolfgang
dc.contributor.authorPuff, Christina
dc.identifier.citationJ Cell Mol Med. 2020;10.1111/jcmm.15585. doi:10.1111/jcmm.15585.en_US
dc.description.abstractSarcomas especially of histiocytic origin often possess a poor prognosis and response to conventional therapies. Interestingly, tumours undergoing mesenchymal to epithelial transition (MET) are often associated with a favourable clinical outcome. This process is characterized by an increased expression of epithelial markers leading to a decreased invasion and metastatic rate. Based on the failure of conventional therapies, viral oncolysis might represent a promising alternative with canine distemper virus (CDV) as a possible candidate. This study hypothesizes that a CDV infection of canine histiocytic sarcoma cells (DH82 cells) triggers the MET process leading to a decreased cellular motility. Immunofluorescence and immunoblotting were used to investigate the expression of epithelial and mesenchymal markers followed by scratch assay and an invasion assay as functional confirmation. Furthermore, microarray data were analysed for genes associated with the MET process, invasion and angiogenesis. CDV-infected cells exhibited an increased expression of epithelial markers such as E-cadherin and cytokeratin 8 compared to controls, indicating a MET process. This was accompanied by a reduced cell motility and invasiveness. Summarized, these results suggest that CDV infection of DH82 cells triggers the MET process by an increased expression of epithelial markers resulting in a decreased cell motility in vitro.en_US
dc.publisherBlackwell Publishingen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.subject3D modellingen_US
dc.subjectcanine distemper virusen_US
dc.subjectcanine histiocytic sarcomaen_US
dc.subjectmesenchymal to epithelial transitionen_US
dc.subjectviral oncolysisen_US
dc.titleMesenchymal to epithelial transition driven by canine distemper virus infection of canine histiocytic sarcoma cells contributes to a reduced cell motility in vitro.en_US
dc.contributor.departmentTWINCORE, Zentrum für experimentelle und klinische Infektionsforschung GmbH,Feodor-Lynen Str. 7, 30625 Hannover, Germany.en_US
dc.identifier.journalJournal of cellular and molecular medicineen_US
dc.source.journaltitleJournal of cellular and molecular medicine

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