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Issue Date
2020-06-12
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Show full item recordAbstract
It has been 10 years since the introduction of modern transposon-insertion sequencing (TIS) methods, which combine genome-wide transposon mutagenesis with high-throughput sequencing to estimate the fitness contribution or essentiality of each genetic component in a bacterial genome. Four TIS variations were published in 2009: transposon sequencing (Tn-Seq), transposon-directed insertion site sequencing (TraDIS), insertion sequencing (INSeq) and high-throughput insertion tracking by deep sequencing (HITS). TIS has since become an important tool for molecular microbiologists, being one of the few genome-wide techniques that directly links phenotype to genotype and ultimately can assign gene function. In this Review, we discuss the recent applications of TIS to answer overarching biological questions. We explore emerging and multidisciplinary methods that build on TIS, with an eye towards future applications.Citation
Nat Rev Genet. 2020;1-15. doi:10.1038/s41576-020-0244-x.Affiliation
HIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Shneider Strasse 2, 97080 Würzburg, Germany.Publisher
Springer NatureJournal
Nature reviews. GeneticsPubMed ID
32533119PubMed Central ID
PMC7291929Additional Links
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7291929/Type
ReviewOther
Language
enEISSN
1471-0064ae974a485f413a2113503eed53cd6c53
10.1038/s41576-020-0244-x
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