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dc.contributor.authorCain, Amy K
dc.contributor.authorBarquist, Lars
dc.contributor.authorGoodman, Andrew L
dc.contributor.authorPaulsen, Ian T
dc.contributor.authorParkhill, Julian
dc.contributor.authorvan Opijnen, Tim
dc.date.accessioned2020-07-30T09:41:24Z
dc.date.available2020-07-30T09:41:24Z
dc.date.issued2020-06-12
dc.identifier.citationNat Rev Genet. 2020;1-15. doi:10.1038/s41576-020-0244-x.en_US
dc.identifier.pmid32533119
dc.identifier.doi10.1038/s41576-020-0244-x
dc.identifier.urihttp://hdl.handle.net/10033/622374
dc.description.abstractIt has been 10 years since the introduction of modern transposon-insertion sequencing (TIS) methods, which combine genome-wide transposon mutagenesis with high-throughput sequencing to estimate the fitness contribution or essentiality of each genetic component in a bacterial genome. Four TIS variations were published in 2009: transposon sequencing (Tn-Seq), transposon-directed insertion site sequencing (TraDIS), insertion sequencing (INSeq) and high-throughput insertion tracking by deep sequencing (HITS). TIS has since become an important tool for molecular microbiologists, being one of the few genome-wide techniques that directly links phenotype to genotype and ultimately can assign gene function. In this Review, we discuss the recent applications of TIS to answer overarching biological questions. We explore emerging and multidisciplinary methods that build on TIS, with an eye towards future applications.en_US
dc.language.isoenen_US
dc.publisherSpringer Natureen_US
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7291929/en_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleA decade of advances in transposon-insertion sequencing.en_US
dc.typeReviewen_US
dc.typeOtheren_US
dc.identifier.eissn1471-0064
dc.contributor.departmentHIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Shneider Strasse 2, 97080 Würzburg, Germany.en_US
dc.identifier.journalNature reviews. Geneticsen_US
dc.identifier.pmcidPMC7291929
refterms.dateFOA2020-07-30T09:41:25Z
dc.source.journaltitleNature reviews. Genetics
dc.source.countryUnited States
dc.source.countryEngland


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