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dc.contributor.authorPirritano, Marcello
dc.contributor.authorZaburannyi, Nestor
dc.contributor.authorGrosser, Katrin
dc.contributor.authorGasparoni, Gilles
dc.contributor.authorMüller, Rolf
dc.contributor.authorSimon, Martin
dc.contributor.authorSchrallhammer, Martina
dc.date.accessioned2020-08-07T11:57:45Z
dc.date.available2020-08-07T11:57:45Z
dc.date.issued2020-06-16
dc.identifier.citationSci Rep. 2020;10(1):9727. Published 2020 Jun 16. doi:10.1038/s41598-020-65894-1.en_US
dc.identifier.pmid32546745
dc.identifier.doi10.1038/s41598-020-65894-1
dc.identifier.urihttp://hdl.handle.net/10033/622394
dc.description.abstractInterest in host-symbiont interactions is continuously increasing, not only due to the growing recognition of the importance of microbiomes. Starting with the detection and description of novel symbionts, attention moves to the molecular consequences and innovations of symbioses. However, molecular analysis requires genomic data which is difficult to obtain from obligate intracellular and uncultivated bacteria. We report the identification of the Caedibacter genome, an obligate symbiont of the ciliate Paramecium. The infection does not only confer the host with the ability to kill other cells but also renders them immune against this effect. We obtained the C. taeniospiralis genome and transcriptome by dual-Seq of DNA and RNA from infected paramecia. Comparison of codon usage and expression level indicates that genes necessary for a specific trait of this symbiosis, i.e. the delivery of an unknown toxin, result from horizontal gene transfer hinting to the relevance of DNA transfer for acquiring new characters. Prediction of secreted proteins of Caedibacter as major agents of contact with the host implies, next to several toxin candidates, a rather uncharacterized secretome which appears to be highly adapted to this symbiosis. Our data provides new insights into the molecular establishment and evolution of this obligate symbiosis and for the pathway characterization of toxicity and immunity.en_US
dc.language.isoenen_US
dc.publisherNPGen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleDual-Seq reveals genome and transcriptome of Caedibacter taeniospiralis, obligate endosymbiont of Paramecium.en_US
dc.typeArticleen_US
dc.identifier.eissn2045-2322
dc.contributor.departmentHIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1 66123 Saarbrücken, Germany.en_US
dc.identifier.journalScientific reportsen_US
dc.relation.embeddedInterest in host-symbiont interactions is continuously increasing, not only due to the growing recognition of the importance of microbiomes. Starting with the detection and description of novel symbionts, attention moves to the molecular consequences and innovations of symbioses. However, molecular analysis requires genomic data which is difficult to obtain from obligate intracellular and uncultivated bacteria. We report the identification of the Caedibacter genome, an obligate symbiont of the ciliate Paramecium. The infection does not only confer the host with the ability to kill other cells but also renders them immune against this effect. We obtained the C. taeniospiralis genome and transcriptome by dual-Seq of DNA and RNA from infected paramecia. Comparison of codon usage and expression level indicates that genes necessary for a specific trait of this symbiosis, i.e. the delivery of an unknown toxin, result from horizontal gene transfer hinting to the relevance of DNA transfer for acquiring new characters. Prediction of secreted proteins of Caedibacter as major agents of contact with the host implies, next to several toxin candidates, a rather uncharacterized secretome which appears to be highly adapted to this symbiosis. Our data provides new insights into the molecular establishment and evolution of this obligate symbiosis and for the pathway characterization of toxicity and immunity.en_US
dc.source.volume10
dc.source.issue1
dc.source.beginpage9727
dc.source.endpage
refterms.dateFOA2020-08-07T11:57:46Z
dc.source.journaltitleScientific reports
dc.source.countryEngland


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