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dc.contributor.authorZhao, Weining
dc.contributor.authorCross, Ashley R
dc.contributor.authorCrowe-McAuliffe, Caillan
dc.contributor.authorWeigert-Munoz, Angela
dc.contributor.authorCsatary, Erika E
dc.contributor.authorSolinski, Amy E
dc.contributor.authorKrysiak, Joanna
dc.contributor.authorGoldberg, Joanna B
dc.contributor.authorWilson, Daniel N
dc.contributor.authorMedina, Eva
dc.contributor.authorWuest, William M
dc.contributor.authorSieber, Stephan A
dc.date.accessioned2020-08-12T13:18:06Z
dc.date.available2020-08-12T13:18:06Z
dc.date.issued2019-05-16
dc.identifier.citationAngew Chem Int Ed Engl. 2019;58(25):8581-8584. doi:10.1002/anie.201903472.en_US
dc.identifier.pmid30969469
dc.identifier.doi10.1002/anie.201903472
dc.identifier.urihttp://hdl.handle.net/10033/622403
dc.description.abstractNatural products represent a rich source of antibiotics that address versatile cellular targets. The deconvolution of their targets via chemical proteomics is often challenged by the introduction of large photocrosslinkers. Here we applied elegaphenone, a largely uncharacterized natural product antibiotic bearing a native benzophenone core scaffold, for affinity-based protein profiling (AfBPP) in Gram-positive and Gram-negative bacteria. This study utilizes the alkynylated natural product scaffold as a probe to uncover intriguing biological interactions with the transcriptional regulator AlgP. Furthermore, proteome profiling of a Pseudomonas aeruginosa AlgP transposon mutant provided unique insights into the mode of action. Elegaphenone enhanced the elimination of intracellular P. aeruginosa in macrophages exposed to sub-inhibitory concentrations of the fluoroquinolone antibiotic norfloxacin.en_US
dc.language.isoenen_US
dc.publisherWiley-VCHen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectPseudomonas aeruginosaen_US
dc.subjectantibioticsen_US
dc.subjectchemical proteomicsen_US
dc.subjectnatural productsen_US
dc.subjectvirulenceen_US
dc.titleThe Natural Product Elegaphenone Potentiates Antibiotic Effects against Pseudomonas aeruginosa.en_US
dc.typeArticleen_US
dc.typeOtheren_US
dc.identifier.eissn1521-3773
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalAngewandte Chemie (International ed. in English)en_US
dc.source.volume58
dc.source.issue25
dc.source.beginpage8581
dc.source.endpage8584
refterms.dateFOA2020-08-12T13:18:08Z
dc.source.journaltitleAngewandte Chemie (International ed. in English)
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryInternational
dc.source.countryGermany


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