Establishment of porcine and human expanded potential stem cells.
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Authors
Gao, XuefeiNowak-Imialek, Monika
Chen, Xi
Chen, Dongsheng
Herrmann, Doris
Ruan, Degong
Chen, Andy Chun Hang
Eckersley-Maslin, Melanie A
Ahmad, Shakil
Lee, Yin Lau
Kobayashi, Toshihiro
Ryan, David
Zhong, Jixing
Zhu, Jiacheng
Wu, Jian
Lan, Guocheng
Petkov, Stoyan
Yang, Jian
Antunes, Liliana
Campos, Lia S
Fu, Beiyuan
Wang, Shengpeng
Yong, Yu
Wang, Xiaomin
Xue, Song-Guo
Ge, Liangpeng
Liu, Zuohua
Huang, Yong
Nie, Tao
Li, Peng
Wu, Donghai
Pei, Duanqing
Zhang, Yi
Lu, Liming
Yang, Fengtang
Kimber, Susan J
Reik, Wolf
Zou, Xiangang
Shang, Zhouchun
Lai, Liangxue
Surani, Azim
Tam, Patrick P L
Ahmed, Asif
Yeung, William Shu Biu
Teichmann, Sarah A
Niemann, Heiner
Liu, Pentao
Issue Date
2019-06-03
Metadata
Show full item recordAbstract
We recently derived mouse expanded potential stem cells (EPSCs) from individual blastomeres by inhibiting the critical molecular pathways that predispose their differentiation. EPSCs had enriched molecular signatures of blastomeres and possessed developmental potency for all embryonic and extra-embryonic cell lineages. Here, we report the derivation of porcine EPSCs, which express key pluripotency genes, are genetically stable, permit genome editing, differentiate to derivatives of the three germ layers in chimeras and produce primordial germ cell-like cells in vitro. Under similar conditions, human embryonic stem cells and induced pluripotent stem cells can be converted, or somatic cells directly reprogrammed, to EPSCs that display the molecular and functional attributes reminiscent of porcine EPSCs. Importantly, trophoblast stem-cell-like cells can be generated from both human and porcine EPSCs. Our pathway-inhibition paradigm thus opens an avenue for generating mammalian pluripotent stem cells, and EPSCs present a unique cellular platform for translational research in biotechnology and regenerative medicine.Citation
Nat Cell Biol. 2019;21(6):687-699. doi:10.1038/s41556-019-0333-2.Affiliation
TWINCORE, Zentrum für experimentelle und klinische Infektionsforschung GmbH,Feodor-Lynen Str. 7, 30625 Hannover, Germany.Publisher
Nature publishing group(NPG)Journal
Nature cell biologyPubMed ID
31160711PubMed Central ID
PMC7035105Additional Links
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7035105/Type
ArticleOther
Language
enEISSN
1476-4679ae974a485f413a2113503eed53cd6c53
10.1038/s41556-019-0333-2
Scopus Count
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The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International
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