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dc.contributor.authorPils, Marina C
dc.contributor.authorKränzler, Katrin
dc.contributor.authorBeyer, Petra
dc.contributor.authorHeise, Ulrike
dc.contributor.authorPasche, Bastian
dc.contributor.authorRiedesel, Hermann
dc.date.accessioned2020-09-02T13:46:07Z
dc.date.available2020-09-02T13:46:07Z
dc.date.issued2018-11-06
dc.identifier.citationJ Am Assoc Lab Anim Sci. 2019;58(1):87-91. doi:10.30802/AALAS-JAALAS-18-000020.en_US
dc.identifier.issn1559-6109
dc.identifier.pmid30401066
dc.identifier.doi10.30802/AALAS-JAALAS-18-000020
dc.identifier.urihttp://hdl.handle.net/10033/622424
dc.description.abstractThe sterilization of potentially infectious animal carcasses is an important biologic safety issue in animal facilities operating as infection or quarantine barriers. However, the literature lacks a validated protocol. Here we describe the validation of an autoclave program suitable for daily use in a small rodent biocontainment unit. We evaluated several procedures for processing mouse carcasses in a standard autoclave. Heat sensors and biologic indicators were implanted inside the peritoneal cavity of dead mice, which were loaded at various densities into IVC cages or metal boxes. Heat sensors revealed broad differences in temperature inside carcasses compared with the autoclave chamber. Achieving the appropriate sterilization temperature was considerably prolonged in carcasses compared with typical laboratory waste material. We show that for 5 cadavers placed well separated inside an IVC, a modified program for mouse cage sterilization using 134 °C for 15 min is suitable. To sterilize approximately 1 kg of carcasses in autoclavable boxes, a period of 6 h is required to reach an effective temperature of 121 °C for 60 min at the center of the waste by using an autoclave program for liquids. In conclusion, we here validated 2 protocols for the sterilization of potentially infectious mouse carcasses, to ensure the application of efficacious procedures.en_US
dc.language.isoenen_US
dc.publisherAmerican Association for Laboratory Animal Scienceen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleValidation of an Autoclave Procedure for Sterilization of Mouse (Mus musculus) Carcasses.en_US
dc.typeArticleen_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalJournal of the American Association for Laboratory Animal Science : JAALASen_US
dc.source.volume58
dc.source.issue1
dc.source.beginpage87
dc.source.endpage91
refterms.dateFOA2020-09-02T13:46:07Z
dc.source.journaltitleJournal of the American Association for Laboratory Animal Science : JAALAS
dc.source.countryUnited States


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