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dc.contributor.authorRungelrath, Viktoria
dc.contributor.authorWeiße, Christine
dc.contributor.authorSchütze, Nicole
dc.contributor.authorMüller, Uwe
dc.contributor.authorMeurer, Marita
dc.contributor.authorRohde, Manfred
dc.contributor.authorSeele, Jana
dc.contributor.authorValentin-Weigand, Peter
dc.contributor.authorKirschfink, Michael
dc.contributor.authorBeineke, Andreas
dc.contributor.authorSchrödl, Wieland
dc.contributor.authorBergmann, René
dc.contributor.authorBaums, Christoph Georg
dc.date.accessioned2020-09-03T10:20:31Z
dc.date.available2020-09-03T10:20:31Z
dc.date.issued2018-08-28
dc.identifier.citationVirulence. 2018;9(1):1314-1337. doi:10.1080/21505594.2018.1496778.en_US
dc.identifier.pmid30001174
dc.identifier.doi10.1080/21505594.2018.1496778
dc.identifier.urihttp://hdl.handle.net/10033/622426
dc.description.abstractStreptococcus suis (S. suis) causes meningitis, arthritis and endocarditis in piglets. The aim of this study was to characterize the IgM degrading enzyme of S. suis (IdeSsuis) and to investigate the role of IgM cleavage in evasion of the classical complement pathway and pathogenesis. Targeted mutagenesis of a cysteine in the putative active center of IdeSsuis abrogated IgM cleavage completely. In contrast to wt rIdeSsuis, point mutated rIdeSsuis_C195S did not reduce complement-mediated hemolysis indicating that complement inhibition by rIdeSsuis depends on the IgM proteolytic activity. A S. suis mutant expressing IdeSsuis_C195S did not reduce IgM labeling, whereas the wt and complemented mutant showed less IgM F(ab')2 and IgM Fc antigen on the surface. IgM cleavage increased survival of S. suis in porcine blood ex vivo and mediated complement evasion as demonstrated by blood survival and C3 deposition assays including the comparative addition of rIdeSsuis and rIdeSsuis_C195S. However, experimental infection of piglets disclosed no significant differences in virulence between S. suis wt and isogenic mutants without IgM cleavage activity. This work revealed for the first time in vivo labeling of S. suis with IgM in the cerebrospinal fluid of piglets with meningitis. In conclusion, this study classifies IdeSsuis as a cysteine protease and emphasizes the role of IgM cleavage for bacterial survival in porcine blood and complement evasion though IgM cleavage is not crucial for the pathogenesis of serotype 2 meningitis.en_US
dc.language.isoenen_US
dc.publisherTaylor & Francisen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectIdeS-family proteaseen_US
dc.subjectIdeSsuisen_US
dc.subjectIgMen_US
dc.subjectStreptococcus suisen_US
dc.subjectcysteine proteaseen_US
dc.subjectopsonizationen_US
dc.titleIgM cleavage by Streptococcus suis reduces IgM bound to the bacterial surface and is a novel complement evasion mechanism.en_US
dc.typeArticleen_US
dc.typeOtheren_US
dc.identifier.eissn2150-5608
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalVirulenceen_US
dc.source.volume9
dc.source.issue1
dc.source.beginpage1314
dc.source.endpage1337
refterms.dateFOA2020-09-03T10:20:32Z
dc.source.journaltitleVirulence
dc.source.countryUnited States


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