Methylene Blue Treatment of Grafts During Cold Ischemia Time Reduces the Risk of Hepatitis C Virus Transmission.
dc.contributor.author | Helfritz, Fabian A | |
dc.contributor.author | Bojkova, Denisa | |
dc.contributor.author | Wanders, Verena | |
dc.contributor.author | Kuklinski, Nina | |
dc.contributor.author | Westhaus, Sandra | |
dc.contributor.author | von Horn, Charlotte | |
dc.contributor.author | Rauen, Ursula | |
dc.contributor.author | Gallinat, Anja | |
dc.contributor.author | Baba, Hideo A | |
dc.contributor.author | Skyschally, Andreas | |
dc.contributor.author | Swoboda, Sandra | |
dc.contributor.author | Kinast, Volker | |
dc.contributor.author | Steinmann, Eike | |
dc.contributor.author | Heusch, Gerd | |
dc.contributor.author | Minor, Thomas | |
dc.contributor.author | Meuleman, Philip | |
dc.contributor.author | Paul, Andreas | |
dc.contributor.author | Ciesek, Sandra | |
dc.date.accessioned | 2020-09-03T12:44:37Z | |
dc.date.available | 2020-09-03T12:44:37Z | |
dc.date.issued | 2018-12-01 | |
dc.identifier.citation | J Infect Dis. 2018;218(11):1711-1721. doi:10.1093/infdis/jiy386. | en_US |
dc.identifier.pmid | 29939277 | |
dc.identifier.doi | 10.1093/infdis/jiy386 | |
dc.identifier.uri | http://hdl.handle.net/10033/622427 | |
dc.description.abstract | Background: Although organ shortage is a rising problem, organs from hepatitis C virus (HCV) ribonucleic acid (RNA)-positive donors are not routinely transplanted in HCV-negative individuals. Because HCV only infects hepatocytes, other organs such as kidneys are merely contaminated with HCV via the blood. In this study, we established a protocol to reduce HCV virions during the cold ischemic time. Methods: Standard virological assays were used to investigate the effect of antivirals, including methylene blue (MB), in different preservation solutions. Kidneys from mini pigs were contaminated with Jc1 or HCV RNA-positive human serum. Afterwards, organs were flushed with MB. Hypothermic machine perfusion was used to optimize reduction of HCV. Results: Three different antivirals were investigated for their ability to inactivate HCV in vitro. Only MB completely inactivated HCV in the presence of all perfusion solutions. Hepatitis C virus-contaminated kidneys from mini pigs were treated with MB and hypothermic machine perfusion without any negative effect on the graft. Human liver-uPA-SCID mice did not establish HCV infection after inoculation with flow through from these kidneys. Conclusions: This proof-of-concept study is a first step to reduce transmission of infectious HCV particles in the transplant setting and might serve as a model for other relevant pathogens. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Oxford Academic | en_US |
dc.rights | Attribution-NonCommercial-ShareAlike 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | * |
dc.title | Methylene Blue Treatment of Grafts During Cold Ischemia Time Reduces the Risk of Hepatitis C Virus Transmission. | en_US |
dc.type | Article | en_US |
dc.identifier.eissn | 1537-6613 | |
dc.contributor.department | TWINCORE, Zentrum für experimentelle und klinische Infektionsforschung GmbH,Feodor-Lynen Str. 7, 30625 Hannover, Germany. | en_US |
dc.identifier.journal | The Journal of infectious diseases | en_US |
dc.source.volume | 218 | |
dc.source.issue | 11 | |
dc.source.beginpage | 1711 | |
dc.source.endpage | 1721 | |
refterms.dateFOA | 2020-09-03T12:44:37Z | |
dc.source.journaltitle | The Journal of infectious diseases | |
dc.source.country | United States |