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dc.contributor.authorHelfritz, Fabian A
dc.contributor.authorBojkova, Denisa
dc.contributor.authorWanders, Verena
dc.contributor.authorKuklinski, Nina
dc.contributor.authorWesthaus, Sandra
dc.contributor.authorvon Horn, Charlotte
dc.contributor.authorRauen, Ursula
dc.contributor.authorGallinat, Anja
dc.contributor.authorBaba, Hideo A
dc.contributor.authorSkyschally, Andreas
dc.contributor.authorSwoboda, Sandra
dc.contributor.authorKinast, Volker
dc.contributor.authorSteinmann, Eike
dc.contributor.authorHeusch, Gerd
dc.contributor.authorMinor, Thomas
dc.contributor.authorMeuleman, Philip
dc.contributor.authorPaul, Andreas
dc.contributor.authorCiesek, Sandra
dc.date.accessioned2020-09-03T12:44:37Z
dc.date.available2020-09-03T12:44:37Z
dc.date.issued2018-12-01
dc.identifier.citationJ Infect Dis. 2018;218(11):1711-1721. doi:10.1093/infdis/jiy386.en_US
dc.identifier.pmid29939277
dc.identifier.doi10.1093/infdis/jiy386
dc.identifier.urihttp://hdl.handle.net/10033/622427
dc.description.abstractBackground: Although organ shortage is a rising problem, organs from hepatitis C virus (HCV) ribonucleic acid (RNA)-positive donors are not routinely transplanted in HCV-negative individuals. Because HCV only infects hepatocytes, other organs such as kidneys are merely contaminated with HCV via the blood. In this study, we established a protocol to reduce HCV virions during the cold ischemic time. Methods: Standard virological assays were used to investigate the effect of antivirals, including methylene blue (MB), in different preservation solutions. Kidneys from mini pigs were contaminated with Jc1 or HCV RNA-positive human serum. Afterwards, organs were flushed with MB. Hypothermic machine perfusion was used to optimize reduction of HCV. Results: Three different antivirals were investigated for their ability to inactivate HCV in vitro. Only MB completely inactivated HCV in the presence of all perfusion solutions. Hepatitis C virus-contaminated kidneys from mini pigs were treated with MB and hypothermic machine perfusion without any negative effect on the graft. Human liver-uPA-SCID mice did not establish HCV infection after inoculation with flow through from these kidneys. Conclusions: This proof-of-concept study is a first step to reduce transmission of infectious HCV particles in the transplant setting and might serve as a model for other relevant pathogens.en_US
dc.language.isoenen_US
dc.publisherOxford Academicen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleMethylene Blue Treatment of Grafts During Cold Ischemia Time Reduces the Risk of Hepatitis C Virus Transmission.en_US
dc.typeArticleen_US
dc.identifier.eissn1537-6613
dc.contributor.departmentTWINCORE, Zentrum für experimentelle und klinische Infektionsforschung GmbH,Feodor-Lynen Str. 7, 30625 Hannover, Germany.en_US
dc.identifier.journalThe Journal of infectious diseasesen_US
dc.source.volume218
dc.source.issue11
dc.source.beginpage1711
dc.source.endpage1721
refterms.dateFOA2020-09-03T12:44:37Z
dc.source.journaltitleThe Journal of infectious diseases
dc.source.countryUnited States


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