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dc.contributor.authorShuai, Hui
dc.contributor.authorMyronovskyi, Maksym
dc.contributor.authorNadmid, Suvd
dc.contributor.authorLuzhetskyy, Andriy
dc.date.accessioned2020-09-11T09:36:26Z
dc.date.available2020-09-11T09:36:26Z
dc.date.issued2020-07-18
dc.identifier.citationBiomolecules. 2020;10(7):E1074. Published 2020 Jul 18. doi:10.3390/biom10071074.en_US
dc.identifier.pmid32708402
dc.identifier.doi10.3390/biom10071074
dc.identifier.urihttp://hdl.handle.net/10033/622439
dc.description.abstractPyrrolopyrimidines are an important class of natural products with a broad spectrum of biological activities, including antibacterial, antifungal, antiviral, anticancer or anti-inflammatory. Here, we present the identification of a biosynthetic gene cluster from the rare actinomycete strain Kutzneria albida DSM 43870, which leads to the production of huimycin, a new member of the pyrrolopyrimidine family of compounds. The huimycin gene cluster was successfully expressed in the heterologous host strain Streptomyces albus Del14. The compound was purified, and its structure was elucidated by means of nuclear magnetic resonance spectroscopy. The minimal huimycin gene cluster was identified through sequence analysis and a series of gene deletion experiments. A model for huimycin biosynthesis is also proposed in this paper.en_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectheterologous expressionen_US
dc.subjectkutzneriaen_US
dc.subjectnucleosideen_US
dc.subjectpyrrolopyrimidinesen_US
dc.subjectsecondary metabolitesen_US
dc.titleIdentification of a Biosynthetic Gene Cluster Responsible for the Production of a New Pyrrolopyrimidine Natural Product-Huimycin.en_US
dc.typeArticleen_US
dc.typeOtheren_US
dc.identifier.eissn2218-273X
dc.contributor.departmentHIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1 66123 Saarbrücken, Germany.en_US
dc.identifier.journalBiomoleculesen_US
dc.source.volume10
dc.source.issue7
refterms.dateFOA2020-09-11T09:36:27Z
dc.source.journaltitleBiomolecules
dc.source.countrySwitzerland


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