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dc.contributor.authorImdahl, Fabian
dc.contributor.authorVafadarnejad, Ehsan
dc.contributor.authorHomberger, Christina
dc.contributor.authorSaliba, Antoine-Emmanuel
dc.contributor.authorVogel, Jörg
dc.date.accessioned2020-09-28T13:29:01Z
dc.date.available2020-09-28T13:29:01Z
dc.date.issued2020-08-17
dc.identifier.citationNat Microbiol. 2020 Oct;5(10):1202-1206. doi: 10.1038/s41564-020-0774-1. Epub 2020 Aug 17. PMID: 32807892.en_US
dc.identifier.pmid32807892
dc.identifier.doi10.1038/s41564-020-0774-1
dc.identifier.urihttp://hdl.handle.net/10033/622488
dc.description.abstractBacteria respond to changes in their environment with specific transcriptional programmes, but even within genetically identical populations these programmes are not homogenously expressed1. Such transcriptional heterogeneity between individual bacteria allows genetically clonal communities to develop a complex array of phenotypes1, examples of which include persisters that resist antibiotic treatment and metabolically specialized cells that emerge under nutrient-limiting conditions2. Fluorescent reporter constructs have played a pivotal role in deciphering heterogeneous gene expression within bacterial populations3 but have been limited to recording the activity of single genes in a few genetically tractable model species, whereas the vast majority of bacteria remain difficult to engineer and/or even to cultivate. Single-cell transcriptomics is revolutionizing the analysis of phenotypic cell-to-cell variation in eukaryotes, but technical hurdles have prevented its robust application to prokaryotes. Here, using an improved poly(A)-independent single-cell RNA-sequencing protocol, we report the faithful capture of growth-dependent gene expression patterns in individual Salmonella and Pseudomonas bacteria across all RNA classes and genomic regions. These transcriptomes provide important reference points for single-cell RNA-sequencing of other bacterial species, mixed microbial communities and host-pathogen interactions.en_US
dc.language.isoenen_US
dc.publisherNature researchen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleSingle-cell RNA-sequencing reports growth-condition-specific global transcriptomes of individual bacteria.en_US
dc.typeArticleen_US
dc.identifier.eissn2058-5276
dc.contributor.departmentHIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Shneider Strasse 2, 97080 Würzburg, Germany.en_US
dc.identifier.journalNature microbiologyen_US
dc.source.volume5
dc.source.issue10
dc.source.beginpage1202
dc.source.endpage1206
dc.source.journaltitleNature microbiology
dc.source.countryEngland


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