Investigating the Function of Cryptic Cytochalasan Cytochrome P450 Monooxygenases Using Combinatorial Biosynthesis.
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Authors
Wang, ChongqingBecker, Kevin
Pfütze, Sebastian
Kuhnert, Eric
Stadler, Marc

Cox, Russell J
Skellam, Elizabeth
Issue Date
2019-10-23
Metadata
Show full item recordAbstract
Tailoring enzymes in cytochalasan biosynthesis are relatively promiscuous. Exploiting this property, we deduced the function of four cryptic cytochrome P450 monooxygenases via heterologous expression of six cytochrome P450-encoding genes, originating from Hypoxylon fragiforme and Pyricularia oryzae, in pyrichalasin H ΔP450 strains. Three cryptic cytochrome P450 enzymes (HffD, HffG, and CYP1) restored pyrichalasin H production in mutant strains, while CYP3 catalyzed a site-selective epoxidation leading to the isolation of three novel cytochalasans.Citation
Org Lett. 2019 Nov 1;21(21):8756-8760. doi: 10.1021/acs.orglett.9b03372.Affiliation
HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.Publisher
ACSJournal
Organic lettersPubMed ID
31644300Type
ArticleOther
Language
enEISSN
1523-7052ae974a485f413a2113503eed53cd6c53
10.1021/acs.orglett.9b03372
Scopus Count
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- Creative Commons
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