Grad-seq shines light on unrecognized RNA and protein complexes in the model bacterium Escherichia coli.
dc.contributor.author | Hör, Jens | |
dc.contributor.author | Di Giorgio, Silvia | |
dc.contributor.author | Gerovac, Milan | |
dc.contributor.author | Venturini, Elisa | |
dc.contributor.author | Förstner, Konrad U | |
dc.contributor.author | Vogel, Jörg | |
dc.date.accessioned | 2020-10-16T11:10:17Z | |
dc.date.available | 2020-10-16T11:10:17Z | |
dc.date.issued | 2020-08-19 | |
dc.identifier.citation | Nucleic Acids Res. 2020 Sep 18;48(16):9301-9319. doi: 10.1093/nar/gkaa676. | en_US |
dc.identifier.pmid | 32813020 | |
dc.identifier.doi | 10.1093/nar/gkaa676 | |
dc.identifier.uri | http://hdl.handle.net/10033/622517 | |
dc.description.abstract | Stable protein complexes, including those formed with RNA, are major building blocks of every living cell. Escherichia coli has been the leading bacterial organism with respect to global protein-protein networks. Yet, there has been no global census of RNA/protein complexes in this model species of microbiology. Here, we performed Grad-seq to establish an RNA/protein complexome, reconstructing sedimentation profiles in a glycerol gradient for ∼85% of all E. coli transcripts and ∼49% of the proteins. These include the majority of small noncoding RNAs (sRNAs) detectable in this bacterium as well as the general sRNA-binding proteins, CsrA, Hfq and ProQ. In presenting use cases for utilization of these RNA and protein maps, we show that a stable association of RyeG with 30S ribosomes gives this seemingly noncoding RNA of prophage origin away as an mRNA of a toxic small protein. Similarly, we show that the broadly conserved uncharacterized protein YggL is a 50S subunit factor in assembled 70S ribosomes. Overall, this study crucially extends our knowledge about the cellular interactome of the primary model bacterium E. coli through providing global RNA/protein complexome information and should facilitate functional discovery in this and related species. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Oxford University Press | en_US |
dc.rights | Attribution-NonCommercial-ShareAlike 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | * |
dc.title | Grad-seq shines light on unrecognized RNA and protein complexes in the model bacterium Escherichia coli. | en_US |
dc.type | Article | en_US |
dc.identifier.eissn | 1362-4962 | |
dc.contributor.department | HIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Shneider Strasse 2, 97080 Würzburg, Germany. | en_US |
dc.identifier.journal | Nucleic acids research | en_US |
dc.source.volume | 48 | |
dc.source.issue | 16 | |
dc.source.beginpage | 9301 | |
dc.source.endpage | 9319 | |
refterms.dateFOA | 2020-10-16T11:10:17Z | |
dc.source.journaltitle | Nucleic acids research | |
dc.source.country | England |