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dc.contributor.authorHör, Jens
dc.contributor.authorDi Giorgio, Silvia
dc.contributor.authorGerovac, Milan
dc.contributor.authorVenturini, Elisa
dc.contributor.authorFörstner, Konrad U
dc.contributor.authorVogel, Jörg
dc.date.accessioned2020-10-16T11:10:17Z
dc.date.available2020-10-16T11:10:17Z
dc.date.issued2020-08-19
dc.identifier.citationNucleic Acids Res. 2020 Sep 18;48(16):9301-9319. doi: 10.1093/nar/gkaa676.en_US
dc.identifier.pmid32813020
dc.identifier.doi10.1093/nar/gkaa676
dc.identifier.urihttp://hdl.handle.net/10033/622517
dc.description.abstractStable protein complexes, including those formed with RNA, are major building blocks of every living cell. Escherichia coli has been the leading bacterial organism with respect to global protein-protein networks. Yet, there has been no global census of RNA/protein complexes in this model species of microbiology. Here, we performed Grad-seq to establish an RNA/protein complexome, reconstructing sedimentation profiles in a glycerol gradient for ∼85% of all E. coli transcripts and ∼49% of the proteins. These include the majority of small noncoding RNAs (sRNAs) detectable in this bacterium as well as the general sRNA-binding proteins, CsrA, Hfq and ProQ. In presenting use cases for utilization of these RNA and protein maps, we show that a stable association of RyeG with 30S ribosomes gives this seemingly noncoding RNA of prophage origin away as an mRNA of a toxic small protein. Similarly, we show that the broadly conserved uncharacterized protein YggL is a 50S subunit factor in assembled 70S ribosomes. Overall, this study crucially extends our knowledge about the cellular interactome of the primary model bacterium E. coli through providing global RNA/protein complexome information and should facilitate functional discovery in this and related species.en_US
dc.language.isoenen_US
dc.publisherOxford University Pressen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleGrad-seq shines light on unrecognized RNA and protein complexes in the model bacterium Escherichia coli.en_US
dc.typeArticleen_US
dc.identifier.eissn1362-4962
dc.contributor.departmentHIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Shneider Strasse 2, 97080 Würzburg, Germany.en_US
dc.identifier.journalNucleic acids researchen_US
dc.source.volume48
dc.source.issue16
dc.source.beginpage9301
dc.source.endpage9319
refterms.dateFOA2020-10-16T11:10:17Z
dc.source.journaltitleNucleic acids research
dc.source.countryEngland


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Attribution-NonCommercial-ShareAlike 4.0 International
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