The Arp2/3 complex is critical for colonisation of the mouse skin by melanoblasts.
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Authors
Papalazarou, VassilisSwaminathan, Karthic
Jaber-Hijazi, Farah
Spence, Heather
Lahmann, Ines
Nixon, Colin
Salmeron-Sanchez, Manuel
Arnold, Hans-Henning
Rottner, Klemens
Machesky, Laura M
Issue Date
2020-10-07
Metadata
Show full item recordAbstract
The Arp2/3 complex is essential for the assembly of branched filamentous actin but its role in physiology and development is surprisingly little understood. Melanoblasts deriving from the neural crest migrate along the developing embryo and traverse the dermis to reach the epidermis colonising the skin and eventually homing within the hair follicles. We have previously established that Rac1 and Cdc42 direct melanoblast migration in vivo We hypothesised that the Arp2/3 complex might be the main downstream effector of these small GTPases. Arp3 depletion in the melanocyte lineage results in severe pigmentation defects in dorsal and ventral regions of the mouse skin. Arp3 null melanoblasts demonstrate proliferation and migration defects and fail to elongate as their wild-type counterparts. Conditional deletion of Arp3 in primary melanocytes causes improper proliferation, spreading, migration and adhesion to extracellular matrix. Collectively, our results suggest that the Arp2/3 complex is absolutely indispensable in the melanocyte lineage in mouse development, and indicate a significant role in developmental processes that require tight regulation of actin-mediated motility.Citation
Development. 2020 Oct 7:dev.194555. doi: 10.1242/dev.194555.Affiliation
HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.Publisher
Company of BiologistsJournal
Development (Cambridge, England)PubMed ID
33028610Type
ArticleLanguage
enEISSN
1477-9129ae974a485f413a2113503eed53cd6c53
10.1242/dev.194555
Scopus Count
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- Creative Commons
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