Host-induced spermidine production in motile triggers phagocytic uptake.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractExploring the complexity of host-pathogen communication is vital to understand why microbes persist within a host, while others are cleared. Here, we employed a dual-sequencing approach to unravel conversational turn-taking of dynamic host-pathogen communications. We demonstrate that upon hitting a host cell, motile Pseudomonas aeruginosa induce a specific gene expression program. This results in the expression of spermidine on the surface, which specifically activates the PIP3-pathway to induce phagocytic uptake into primary or immortalized murine cells. Non-motile bacteria are more immunogenic due to a lower expression of arnT upon host-cell contact, but do not produce spermidine and are phagocytosed less. We demonstrate that not only the presence of pathogen inherent molecular patterns induces immune responses, but that bacterial motility is linked to a host-cell-induced expression of additional immune modulators. Our results emphasize on the value of integrating microbiological and immunological findings to unravel complex and dynamic host-pathogen interactions.
CitationElife. 2020 Sep 22;9:e55744. doi: 10.7554/eLife.55744.
AffiliationHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.
The following license files are associated with this item:
- Creative Commons
- Identification of cell-surface glycans that mediate motility-dependent binding and internalization of Pseudomonas aeruginosa by phagocytes.
- Authors: Sanchez H, Hopkins D, Demirdjian S, Gutierrez C, O'Toole GA, Neelamegham S, Berwin B
- Issue date: 2021 Mar
- Pseudomonas aeruginosa flagellar motility activates the phagocyte PI3K/Akt pathway to induce phagocytic engulfment.
- Authors: Lovewell RR, Hayes SM, O'Toole GA, Berwin B
- Issue date: 2014 Apr 1
- Phosphatidylinositol-(3,4,5)-Trisphosphate Induces Phagocytosis of Nonmotile Pseudomonas aeruginosa.
- Authors: Demirdjian S, Hopkins D, Sanchez H, Libre M, Gerber SA, Berwin B
- Issue date: 2018 Aug
- Distinct Contributions of CD18 Integrins for Binding and Phagocytic Internalization of Pseudomonas aeruginosa.
- Authors: Demirdjian S, Hopkins D, Cumbal N, Lefort CT, Berwin B
- Issue date: 2020 Apr 20
- Reversible inhibition of flagella formation after specific inhibition of spermidine synthesis by dicyclohexylamine in Pseudomonas aeruginosa.
- Authors: Paulin L, Lindberg LA, Pösö H
- Issue date: 1986