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dc.contributor.authorSingh, Avishek Kumar
dc.contributor.authorRooge, Sheetalnath Babasaheb
dc.contributor.authorVarshney, Aditi
dc.contributor.authorVasudevan, Madavan
dc.contributor.authorKumar, Manoj
dc.contributor.authorGeffers, Robert
dc.contributor.authorKumar, Vijay
dc.contributor.authorSarin, Shiv Kumar
dc.date.accessioned2020-11-08T10:46:00Z
dc.date.available2020-11-08T10:46:00Z
dc.date.issued2020-10-27
dc.identifier.citationJ Med Virol. 2020 Oct 27. doi: 10.1002/jmv.26629. Epub ahead of print.en_US
dc.identifier.pmid33107616
dc.identifier.doi10.1002/jmv.26629
dc.identifier.urihttp://hdl.handle.net/10033/622563
dc.description.abstractThe uptake or expression of hepatitis B virus proteins by Dendritic cells (DCs) is considered important for disease outcome. Differential expression of microRNA may have a role in viral persistence and hepatocellular injury. The miRNA expression was investigated by microarray in DCs from different stages of HBV infection and liver disease viz., immune active (IA; n=20); low replicative (LR; n=20); HBeAg negative (n=20); acute viral hepatitis (AVH, n=20) and healthy controls (n=20). miRNA levels were analyzed by unsupervised hierarchical clustering and principal component analyses and validated by qPCR. The miRNA-mRNA regulatory networks identified 19 miRNAs and 12 target gene interactions in MHC and other immune pathways. miR-2278, miR-615-3p and miR-3681-3p were down-regulated in IA group compared to healthy control, miR-152-3p and miR-3613-3p in LR group compared to IA group and miR-152-3p and miR-503-3p in HBe negative compared to LR group. However, miR-7-1-1-3p, miR-192-5p, miR-195-5p and miR-32-5p in LR, miR-342-3p and miR-940 in HBe negative, and miR-34a-5p, miR-130b-3p, miR-221-3p, miR-320a, miR-324-5p and miR-484 in AVH were up-regulated. Further, qPCR confirmed changes in miRNA levels and their target genes associated with antigen processing and presentation. Thus, a deregulated network of miRNAs-mRNAs in DCs seems responsible for impaired immune response during HBV pathogenesis. This article is protected by copyright. All rights reserved.en_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectHBe antigenen_US
dc.subjectMicroarrayen_US
dc.subjectRT-qPCRen_US
dc.subjectacute viral hepatitisen_US
dc.subjectantigen presentationen_US
dc.subjectchronic hepatitis Ben_US
dc.titleIdentification of miRNAs associated with dendritic cell dysfunction during Acute and Chronic Hepatitis B virus infection.en_US
dc.typeArticleen_US
dc.identifier.eissn1096-9071
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalJournal of medical virologyen_US
dc.source.journaltitleJournal of medical virology
dc.source.countryUnited States


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