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dc.contributor.authorWicke, Laura
dc.contributor.authorPonath, Falk
dc.contributor.authorCoppens, Lucas
dc.contributor.authorGerovac, Milan
dc.contributor.authorLavigne, Rob
dc.contributor.authorVogel, Jörg
dc.date.accessioned2020-11-09T14:37:23Z
dc.date.available2020-11-09T14:37:23Z
dc.date.issued2020-10-25
dc.identifier.citationRNA Biol. 2020 Oct 25:1-12. doi: 10.1080/15476286.2020.1827785.en_US
dc.identifier.pmid33103565
dc.identifier.doi10.1080/15476286.2020.1827785
dc.identifier.urihttp://hdl.handle.net/10033/622565
dc.description.abstractAs part of the ongoing renaissance of phage biology, more phage genomes are becoming available through DNA sequencing. However, our understanding of the transcriptome architecture that allows these genomes to be expressed during host infection is generally poor. Transcription start sites (TSSs) and operons have been mapped for very few phages, and an annotated global RNA map of a phage - alone or together with its infected host - is not available at all. Here, we applied differential RNA-seq (dRNA-seq) to study the early, host takeover phase of infection by assessing the transcriptome structure of Pseudomonas aeruginosa jumbo phage ɸKZ, a model phage for viral genetics and structural research. This map substantially expands the number of early expressed viral genes, defining TSSs that are active ten minutes after ɸKZ infection. Simultaneously, we record gene expression changes in the host transcriptome during this critical metabolism conversion. In addition to previously reported upregulation of genes associated with amino acid metabolism, we observe strong activation of genes with functions in biofilm formation (cdrAB) and iron storage (bfrB), as well as an activation of the antitoxin ParD. Conversely, ɸKZ infection rapidly down-regulates complexes IV and V of oxidative phosphorylation (atpCDGHF and cyoABCDE). Taken together, our data provide new insights into the transcriptional organization and infection process of the giant bacteriophage ɸKZ and adds a framework for the genome-wide transcriptomic analysis of phage-host interactions.en_US
dc.language.isoenen_US
dc.publisherTaylor & Francisen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectPseudomonas aeruginosaen_US
dc.subjectBacteriophage ɸKZen_US
dc.subjectdRNA-seqen_US
dc.subjectdifferential expressionen_US
dc.subjectphage-host interactionen_US
dc.subjecttranscription start siteen_US
dc.titleIntroducing differential RNA-seq mapping to track the early infection phase for phage ɸKZ.en_US
dc.typeArticleen_US
dc.identifier.eissn1555-8584
dc.contributor.departmentHIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Shneider Strasse 2, 97080 Würzburg, Germany.en_US
dc.identifier.journalRNA biologyen_US
dc.source.beginpage1
dc.source.endpage12
refterms.dateFOA2020-11-09T14:37:23Z
dc.source.journaltitleRNA biology
dc.source.countryUnited States


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