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dc.contributor.authorRahim, Muhammad Imran
dc.contributor.authorWinkel, Andreas
dc.contributor.authorLienenklaus, Stefan
dc.contributor.authorStumpp, Nico S
dc.contributor.authorSzafrański, Szymon P
dc.contributor.authorKommerein, Nadine
dc.contributor.authorWillbold, Elmar
dc.contributor.authorReifenrath, Janin
dc.contributor.authorMueller, Peter P
dc.contributor.authorEisenburger, Michael
dc.contributor.authorStiesch, Meike
dc.date.accessioned2020-11-10T13:41:59Z
dc.date.available2020-11-10T13:41:59Z
dc.date.issued2020-10-21
dc.identifier.citationMicroorganisms. 2020 Oct 21;8(10):1624. doi: 10.3390/microorganisms8101624. PMID: 33096869.en_US
dc.identifier.issn2076-2607
dc.identifier.pmid33096869
dc.identifier.doi10.3390/microorganisms8101624
dc.identifier.urihttp://hdl.handle.net/10033/622570
dc.description.abstractThe performance of biomaterials is often compromised by bacterial infections and subsequent inflammation. So far, the conventional analysis of inflammatory processes in vivo involves time-consuming histology and biochemical assays. The present study employed a mouse model where interferon beta (IFN-β) is monitored as a marker for non-invasive rapid detection of inflammation in implant-related infections. The mouse model comprises subcutaneous implantation of morphologically modified titanium, followed by experimental infections with four taxonomically diverse oral bacteria: Streptococcus oralis, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis and Treponema denticola (as mono culture or selected mixed-culture). IFN-β expression increased upon infections depending on the type of pathogen and was prolonged by the presence of the implant. IFN-β expression kinetics reduced with two mixed species infections when compared with the single species. Histological and confocal microscopy confirmed pathogen-specific infiltration of inflammatory cells at the implant-tissue interface. This was observed mainly in the vicinity of infected implants and was, in contrast to interferon expression, higher in infections with dual species. In summary, this non-invasive mouse model can be used to quantify longitudinally host inflammation in real time and suggests that the polymicrobial character of infection, highly relevant to clinical situations, has complex effects on host immunity.en_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectanimal modelen_US
dc.subjectbioluminescenceen_US
dc.subjectbiomaterial-associated infectionsen_US
dc.subjectimmune responseen_US
dc.subjectnon-invasive in vivo imagingen_US
dc.subjectperiodontal pathogensen_US
dc.subjecttype I interferonen_US
dc.titleNon-Invasive Luciferase Imaging of Type I Interferon Induction in a Transgenic Mouse Model of Biomaterial Associated Bacterial Infections: Microbial Specificity and Inter-Bacterial Species Interactions.en_US
dc.typeArticleen_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalMicroorganismsen_US
dc.source.volume8
dc.source.issue10
refterms.dateFOA2020-11-10T13:41:59Z
dc.source.journaltitleMicroorganisms
dc.source.countrySwitzerland


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