Analysis of the organization and expression patterns of the convergent pseudomonas aeruginosa lasr/rsal gene pair uncovers mutual influence.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractThe two adjacent genes encoding the major Pseudomonas aeruginosa quorum sensing regulator, LasR, and its opponent, RsaL, overlap in their coding 3´ends and produce mRNA transcripts with long untranslated 3´ends that overlap with the sense transcripts of the gene on the opposing DNA strand. In this study, we evaluated whether the overlapping genes are involved in mutual regulatory events and studied interference by natural antisense transcripts. We introduced various gene expression constructs into a P. aeruginosa PA14 lasR/rsaL double deletion mutant, and found that although complementary RNA is produced, this does not interfere with the sense gene expression levels of lasR and rsaL and does not have functional consequences on down-stream gene regulation. Nevertheless, expression of lasR, but not of rsaL, was shown to be enhanced if transcription was terminated at the end of the respective gene so that no overlapping transcription was allowed. Our data indicate that the natural organization with a partial overlap at the 3´ends of the lasR/rsaL genes gives rise to a system of checks and balances to prevent dominant and unilateral control by LasR over the RsaL transcriptional regulator of opposing function.
CitationMol Microbiol. 2020 Oct 19. doi: 10.1111/mmi.14628. Epub ahead of print.
AffiliationHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.
The following license files are associated with this item:
- Creative Commons
- The multi-output incoherent feedforward loop constituted by the transcriptional regulators LasR and RsaL confers robustness to a subset of quorum sensing genes in Pseudomonas aeruginosa.
- Authors: Bondí R, Longo F, Messina M, D'Angelo F, Visca P, Leoni L, Rampioni G
- Issue date: 2017 Jun 1
- RsaL provides quorum sensing homeostasis and functions as a global regulator of gene expression in Pseudomonas aeruginosa.
- Authors: Rampioni G, Schuster M, Greenberg EP, Bertani I, Grasso M, Venturi V, Zennaro E, Leoni L
- Issue date: 2007 Dec
- RsaL, a novel repressor of virulence gene expression in Pseudomonas aeruginosa.
- Authors: de Kievit T, Seed PC, Nezezon J, Passador L, Iglewski BH
- Issue date: 1999 Apr
- Mechanisms of RsaL mediated tolerance to ciprofloxacin and carbenicillin in Pseudomonas aeruginosa.
- Authors: Fan Z, Xu C, Pan X, Dong Y, Ren H, Jin Y, Bai F, Cheng Z, Jin S, Wu W
- Issue date: 2019 Feb
- The quorum-sensing negative regulator RsaL of Pseudomonas aeruginosa binds to the lasI promoter.
- Authors: Rampioni G, Bertani I, Zennaro E, Polticelli F, Venturi V, Leoni L
- Issue date: 2006 Jan