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dc.contributor.authorSchinner, Silvia
dc.contributor.authorPreusse, Matthias
dc.contributor.authorKesthely, Christopher
dc.contributor.authorHäussler, Susanne
dc.date.accessioned2020-11-10T15:12:36Z
dc.date.available2020-11-10T15:12:36Z
dc.date.issued2020-10-19
dc.identifier.citationMol Microbiol. 2020 Oct 19. doi: 10.1111/mmi.14628. Epub ahead of print.en_US
dc.identifier.pmid33073409
dc.identifier.doi10.1111/mmi.14628
dc.identifier.urihttp://hdl.handle.net/10033/622572
dc.description.abstractThe two adjacent genes encoding the major Pseudomonas aeruginosa quorum sensing regulator, LasR, and its opponent, RsaL, overlap in their coding 3´ends and produce mRNA transcripts with long untranslated 3´ends that overlap with the sense transcripts of the gene on the opposing DNA strand. In this study, we evaluated whether the overlapping genes are involved in mutual regulatory events and studied interference by natural antisense transcripts. We introduced various gene expression constructs into a P. aeruginosa PA14 lasR/rsaL double deletion mutant, and found that although complementary RNA is produced, this does not interfere with the sense gene expression levels of lasR and rsaL and does not have functional consequences on down-stream gene regulation. Nevertheless, expression of lasR, but not of rsaL, was shown to be enhanced if transcription was terminated at the end of the respective gene so that no overlapping transcription was allowed. Our data indicate that the natural organization with a partial overlap at the 3´ends of the lasR/rsaL genes gives rise to a system of checks and balances to prevent dominant and unilateral control by LasR over the RsaL transcriptional regulator of opposing function.en_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relationinfo:eu-repo/grantAgreement/EC/H2020/ 724290en_US
dc.rightsembargoedAccessen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectPseudomonas aeruginosaen_US
dc.subjectlasRen_US
dc.subjectrsaLen_US
dc.subjecttranscriptional interferenceen_US
dc.titleAnalysis of the organization and expression patterns of the convergent pseudomonas aeruginosa lasr/rsal gene pair uncovers mutual influence.en_US
dc.typeArticleen_US
dc.identifier.eissn1365-2958
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalMolecular microbiologyen_US
dc.source.journaltitleMolecular microbiology
dc.source.countryEngland


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