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dc.contributor.authorLeber, Christopher A
dc.contributor.authorNaman, C Benjamin
dc.contributor.authorKeller, Lena
dc.contributor.authorAlmaliti, Jehad
dc.contributor.authorCaro-Diaz, Eduardo J E
dc.contributor.authorGlukhov, Evgenia
dc.contributor.authorJoseph, Valsamma
dc.contributor.authorSajeevan, T P
dc.contributor.authorReyes, Andres Joshua
dc.contributor.authorBiggs, Jason S
dc.contributor.authorLi, Te
dc.contributor.authorYuan, Ye
dc.contributor.authorHe, Shan
dc.contributor.authorYan, Xiaojun
dc.contributor.authorGerwick, William H
dc.date.accessioned2020-11-12T14:14:13Z
dc.date.available2020-11-12T14:14:13Z
dc.date.issued2020-10-14
dc.identifier.citationMar Drugs. 2020 Oct 14;18(10):515. doi: 10.3390/md18100515.en_US
dc.identifier.pmid33066480
dc.identifier.doi10.3390/md18100515
dc.identifier.urihttp://hdl.handle.net/10033/622577
dc.description.abstractThe tropical marine cyanobacterium Moorena bouillonii occupies a large geographic range across the Indian and Western Tropical Pacific Oceans and is a prolific producer of structurally unique and biologically active natural products. An ensemble of computational approaches, including the creation of the ORCA (Objective Relational Comparative Analysis) pipeline for flexible MS1 feature detection and multivariate analyses, were used to analyze various M. bouillonii samples. The observed chemogeographic patterns suggested the production of regionally specific natural products by M. bouillonii. Analyzing the drivers of these chemogeographic patterns allowed for the identification, targeted isolation, and structure elucidation of a regionally specific natural product, doscadenamide A (1). Analyses of MS2 fragmentation patterns further revealed this natural product to be part of an extensive family of herein annotated, proposed natural structural analogs (doscadenamides B-J, 2-10); the ensemble of structures reflect a combinatorial biosynthesis using nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) components. Compound 1 displayed synergistic in vitro cancer cell cytotoxicity when administered with lipopolysaccharide (LPS). These discoveries illustrate the utility in leveraging chemogeographic patterns for prioritizing natural product discovery efforts.en_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectMoorena bouilloniien_US
dc.subjectchemogeographyen_US
dc.subjectmarine natural productsen_US
dc.subjectmetabolomicsen_US
dc.titleApplying a Chemogeographic Strategy for Natural Product Discovery from the Marine Cyanobacterium .en_US
dc.typeArticleen_US
dc.identifier.eissn1660-3397
dc.contributor.departmentHIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1 66123 Saarbrücken, Germany.en_US
dc.identifier.journalMarine drugsen_US
dc.source.volume18
dc.source.issue10
refterms.dateFOA2020-11-12T14:14:14Z
dc.source.journaltitleMarine drugs
dc.source.countrySwitzerland


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