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dc.contributor.authorKhosravi Babadi, Z
dc.contributor.authorEbrahimipour, G
dc.contributor.authorWink, J
dc.contributor.authorNarmani, A
dc.contributor.authorRisdian, C
dc.date.accessioned2020-11-13T14:10:40Z
dc.date.available2020-11-13T14:10:40Z
dc.date.issued2020-10-15
dc.identifier.citationLett Appl Microbiol. 2020 Oct 15. doi: 10.1111/lam.13415. Epub ahead of print.en_US
dc.identifier.pmid33058293
dc.identifier.doi10.1111/lam.13415
dc.identifier.urihttp://hdl.handle.net/10033/622581
dc.description.abstractIn this study, strain Streptomyces sp. Act4Zk was isolated based on a method developed for the isolation of myxobacteria. Due to the low efficiency of the majority of conventional DNA extraction techniques, for molecular identification of the strain Streptomyces sp. Act4Zk, a new technique for DNA extraction of Actinobacteria was developed. In order to explore potential bioactivities of the strain, extracts of the fermented broth culture were prepared by an organic solvent (i.e. ethyl acetate) extraction method using. These ethyl acetate extracts were subjected to HPLC fractionation against standard micro-organisms, followed by LC/MS analysis. Based on morphological, physiological, biochemical and 16S rRNA gene sequence data, strain Streptomyces sp. Act4Zk is likely to be a new species of Streptomyces, close to Streptomyces genecies and Streptomyces roseolilacinus. Antimicrobial assay indicated high antifungal activity as well as antibacterial activity against Mycobacterium smegmatis and Gram-positive bacteria for the new strain. HPLC and LC/MS analyses of the extracts led to the identification of three different compounds and confirmed our hypothesis that the interesting species of the genus Streptomyces being a good producer of staurosporine and some derivatives.en_US
dc.language.isoenen_US
dc.publisherSociety for Applied Microbiologyen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectStreptomycesen_US
dc.subjectDNA extraction methoden_US
dc.subjectStausporineen_US
dc.subjectantibacterial activityen_US
dc.subjectantifungal activityen_US
dc.titleIsolation and identification of Streptomyces sp. Act4Zk, a good producer of Staurosporine and some derivatives.en_US
dc.typeArticleen_US
dc.identifier.eissn1472-765X
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalLetters in applied microbiologyen_US
dc.source.journaltitleLetters in applied microbiology
dc.source.countryEngland


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