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dc.contributor.authorLasch, Constanze
dc.contributor.authorGummerlich, Nils
dc.contributor.authorMyronovskyi, Maksym
dc.contributor.authorPalusczak, Anja
dc.contributor.authorZapp, Josef
dc.contributor.authorLuzhetskyy, Andriy
dc.date.accessioned2020-11-13T15:03:59Z
dc.date.available2020-11-13T15:03:59Z
dc.date.issued2020-10-09
dc.identifier.citationMolecules. 2020 Oct 9;25(20):4594. doi: 10.3390/molecules25204594.en_US
dc.identifier.pmid33050154
dc.identifier.doi10.3390/molecules25204594
dc.identifier.urihttp://hdl.handle.net/10033/622582
dc.description.abstractNatural products are a valuable source of biologically active compounds with potential applications in medicine and agriculture. Unprecedented scaffold diversity of natural products and biocatalysts from their biosynthetic pathways are of fundamental importance. Heterologous expression and refactoring of natural product biosynthetic pathways are generally regarded as a promising approach to discover new secondary metabolites of microbial origin. Here, we present the identification of a new group of alkylresorcinols after transcriptional activation and heterologous expression of the type III polyketide synthase of Micromonospora endolithica. The most abundant compounds loseolamycins A1 and A2 have been purified and their structures were elucidated by NMR. Loseolamycins contain an unusual branched hydroxylated aliphatic chain which is provided by the host metabolism and is incorporated as a starter fatty acid unit. The isolated loseolamycins show activity against gram-positive bacteria and inhibit the growth of the monocot weed Agrostis stolonifera in a germination assay. The biosynthetic pathway leading to the production of loseolamycins is proposed in this paper.en_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectMicromonospora endolithicaen_US
dc.subjectStreptomyces albus Del14en_US
dc.subjectalkylresorcinolen_US
dc.subjectbiosynthetic geneen_US
dc.subjectheterologous expressionen_US
dc.subjecttype III polyketide synthaseen_US
dc.titleLoseolamycins: A Group of New Bioactive Alkylresorcinols Produced after Heterologous Expression of a Type III PKS from micromonospora endolithica.en_US
dc.typeArticleen_US
dc.identifier.eissn1420-3049
dc.contributor.departmentHIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1 66123 Saarbrücken, Germany.en_US
dc.identifier.journalMolecules (Basel, Switzerland)en_US
dc.source.volume25
dc.source.issue20
refterms.dateFOA2020-11-13T15:04:00Z
dc.source.journaltitleMolecules (Basel, Switzerland)
dc.source.countrySwitzerland


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