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dc.contributor.authorDepke, Tobias
dc.contributor.authorHäussler, Susanne
dc.contributor.authorBrönstrup, Mark
dc.date.accessioned2020-11-13T15:10:03Z
dc.date.available2020-11-13T15:10:03Z
dc.date.issued2020-10-18
dc.identifier.citationMetabolites. 2020 Oct 18;10(10):417. doi: 10.3390/metabo10100417.en_US
dc.identifier.issn2218-1989
dc.identifier.pmid33080992
dc.identifier.doi10.3390/metabo10100417
dc.identifier.urihttp://hdl.handle.net/10033/622583
dc.description.abstractPseudomonas aeruginosa is one of the most important nosocomial pathogens and understanding its virulence is the key to effective control of P. aeruginosa infections. The regulatory network governing virulence factor production in P. aeruginosa is exceptionally complex. Previous studies have shown that the peptide chain release factor methyltransferase PrmC plays an important role in bacterial pathogenicity. Yet, the underlying molecular mechanism is incompletely understood. In this study, we used untargeted liquid and gas chromatography coupled to mass spectrometry to characterise the metabolome of a prmC defective P. aeruginosa PA14 strain in comparison with the corresponding strain complemented with prmC in trans. The comprehensive metabolomics data provided new insight into the influence of prmC on virulence and metabolism. prmC deficiency had broad effects on the endo- and exometabolome of P. aeruginosa PA14, with a marked decrease of the levels of aromatic compounds accompanied by reduced precursor supply from the shikimate pathway. Furthermore, a pronounced decrease of phenazine production was observed as well as lower abundance of alkylquinolones. Unexpectedly, the metabolomics data showed no prmC-dependent effect on rhamnolipid production and an increase in pyochelin levels. A putative virulence biomarker identified in a previous study was significantly less abundant in the prmC deficient strain.en_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectPrmCen_US
dc.subjectPseudomonas aeruginosaen_US
dc.subjectmetabolomicsen_US
dc.subjectvirulenceen_US
dc.titleThe Peptide Chain Release Factor Methyltransferase PrmC Influences the Pseudomonas aeruginosa PA14 Endo- and Exometabolome.en_US
dc.typeArticleen_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalMetabolitesen_US
dc.source.volume10
dc.source.issue10
refterms.dateFOA2020-11-13T15:10:04Z
dc.source.journaltitleMetabolites
dc.source.countrySwitzerland


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