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dc.contributor.authorStressmann, Franziska A
dc.contributor.authorBernal-Bayard, Joaquín
dc.contributor.authorPerez-Pascual, David
dc.contributor.authorAudrain, Bianca
dc.contributor.authorRendueles, Olaya
dc.contributor.authorBriolat, Valérie
dc.contributor.authorBruchmann, Sebastian
dc.contributor.authorVolant, Stevenn
dc.contributor.authorGhozlane, Amine
dc.contributor.authorHäussler, Susanne
dc.contributor.authorDuchaud, Eric
dc.contributor.authorLevraud, Jean-Pierre
dc.contributor.authorGhigo, Jean-Marc
dc.date.accessioned2020-11-16T13:34:09Z
dc.date.available2020-11-16T13:34:09Z
dc.date.issued2020-10-19
dc.identifier.citationISME J. 2020 Oct 19. doi: 10.1038/s41396-020-00807-8. Epub ahead of print.en_US
dc.identifier.pmid33077888
dc.identifier.doi10.1038/s41396-020-00807-8
dc.identifier.urihttp://hdl.handle.net/10033/622585
dc.description.abstractThe long-known resistance to pathogens provided by host-associated microbiota fostered the notion that adding protective bacteria could prevent or attenuate infection. However, the identification of endogenous or exogenous bacteria conferring such protection is often hindered by the complexity of host microbial communities. Here, we used zebrafish and the fish pathogen Flavobacterium columnare as a model system to study the determinants of microbiota-associated colonization resistance. We compared infection susceptibility in germ-free, conventional and reconventionalized larvae and showed that a consortium of 10 culturable bacterial species are sufficient to protect zebrafish. Whereas survival to F. columnare infection does not rely on host innate immunity, we used antibiotic dysbiosis to alter zebrafish microbiota composition, leading to the identification of two different protection strategies. We first identified that the bacterium Chryseobacterium massiliae individually protects both larvae and adult zebrafish. We also showed that an assembly of 9 endogenous zebrafish species that do not otherwise protect individually confer a community-level resistance to infection. Our study therefore provides a rational approach to identify key endogenous protecting bacteria and promising candidates to engineer resilient microbial communities. It also shows how direct experimental analysis of colonization resistance in low-complexity in vivo models can reveal unsuspected ecological strategies at play in microbiota-based protection against pathogens.en_US
dc.language.isoenen_US
dc.publisherSpringer Natureen_US
dc.relationinfo:eu-repo/grantAgreement/EC/H2020/ 842629en_US
dc.rightsopenAccessen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleMining zebrafish microbiota reveals key community-level resistance against fish pathogen infection.en_US
dc.typeArticleen_US
dc.identifier.eissn1751-7370
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalThe ISME journalen_US
refterms.dateFOA2020-11-16T13:34:10Z
dc.source.journaltitleThe ISME journal
dc.source.countryEngland


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