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dc.contributor.authorZielińska, Aleksandra
dc.contributor.authorSavietto, Abigail
dc.contributor.authorde Sousa Borges, Anabela
dc.contributor.authorMartinez, Denis
dc.contributor.authorBerbon, Melanie
dc.contributor.authorRoelofsen, Joël R
dc.contributor.authorHartman, Alwin M
dc.contributor.authorde Boer, Rinse
dc.contributor.authorVan der Klei, Ida J
dc.contributor.authorHirsch, Anna Kh
dc.contributor.authorHabenstein, Birgit
dc.contributor.authorBramkamp, Marc
dc.contributor.authorScheffers, Dirk-Jan
dc.date.accessioned2020-11-18T16:09:29Z
dc.date.available2020-11-18T16:09:29Z
dc.date.issued2020-07-14
dc.identifier.citationElife. 2020 Jul 14;9:e57179. doi: 10.7554/eLife.57179.en_US
dc.identifier.pmid32662773
dc.identifier.doi10.7554/eLife.57179
dc.identifier.urihttp://hdl.handle.net/10033/622596
dc.description.abstractEvery living cell is enclosed by a flexible membrane made of molecules known as phospholipids, which protects the cell from harmful chemicals and other threats. In bacteria and some other organisms, a rigid structure known as the cell wall sits just outside of the membrane and determines the cell’s shape. There are several proteins in the membrane of bacteria that allow the cell to grow by assembling new pieces of the cell wall. To ensure these proteins expand the cell wall at the right locations, another protein known as MreB moves and organizes them to the appropriate place in the membrane and controls their activity. Previous studies have found that another class of proteins called flotillins are involved in arranging proteins and phospholipid molecules within membranes. Bacteria lacking these proteins do not grow properly and are unable to maintain their normal shape. However, the precise role of the flotillins remained unclear. Here, Zielińska, Savietto et al. used microscopy approaches to study flotillins in a bacterium known as Bacillus subtilis. The experiments found that, in the presence of flotillins, MreB moved around the membrane more quickly (suggesting it was more active) than when no flotillins were present. Similar results were observed when bacterial cells lacking flotillins were treated with a chemical that made membranes more ‘fluid’ – that is, made it easier for the molecules within the membrane to travel around. Further experiments found that flotillins allowed the phospholipid molecules within an artificial membrane to move around more freely, which increases the fluidity of the membrane. These findings suggest that flotillins make the membranes of bacterial cells more fluid to help cells expand their walls and perform several other processes. Understanding how bacteria control the components of their membranes will further our understanding of how many currently available antibiotics work and may potentially lead to the design of new antibiotics in the future.en_US
dc.language.isoenen_US
dc.publishereLife Sciences Publications, Ltd.en_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectB. subtilisen_US
dc.subjectbacteriaen_US
dc.subjectcell biologyen_US
dc.subjectfunctional membrane microdomainen_US
dc.subjectinfectious diseaseen_US
dc.subjectlipid raftsen_US
dc.subjectmembrane organisationen_US
dc.subjectmicrobiologyen_US
dc.subjectpenicillin binding proteinen_US
dc.titleFlotillin-mediated membrane fluidity controls peptidoglycan synthesis and MreB movement.en_US
dc.typeArticleen_US
dc.identifier.eissn2050-084X
dc.contributor.departmentHIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1 66123 Saarbrücken, Germany.en_US
dc.identifier.journaleLifeen_US
dc.source.volume9
refterms.dateFOA2020-11-18T16:09:30Z
dc.source.journaltitleeLife
dc.source.countryInternational
dc.source.countryInternational
dc.source.countryInternational
dc.source.countryInternational
dc.source.countryInternational
dc.source.countryInternational
dc.source.countryInternational
dc.source.countryEngland


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