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dc.contributor.authorReimer, Dorothea
dc.contributor.authorMeyer-Hermann, Michael
dc.contributor.authorRakhymzhan, Asylkhan
dc.contributor.authorSteinmetz, Tobit
dc.contributor.authorTripal, Philipp
dc.contributor.authorThomas, Jana
dc.contributor.authorBoettcher, Martin
dc.contributor.authorMougiakakos, Dimitrios
dc.contributor.authorSchulz, Sebastian R
dc.contributor.authorUrbanczyk, Sophia
dc.contributor.authorHauser, Anja E
dc.contributor.authorNiesner, Raluca A
dc.contributor.authorMielenz, Dirk
dc.date.accessioned2020-11-19T15:12:26Z
dc.date.available2020-11-19T15:12:26Z
dc.date.issued2020-08-11
dc.identifier.citationCell Rep. 2020 Aug 11;32(6):108030. doi: 10.1016/j.celrep.2020.108030.en_US
dc.identifier.pmid32783949
dc.identifier.doi10.1016/j.celrep.2020.108030
dc.identifier.urihttp://hdl.handle.net/10033/622601
dc.description.abstractPlasma cells secreting affinity-matured antibodies develop in germinal centers (GCs), where B cells migrate persistently and directionally over defined periods of time. How modes of GC B cell migration influence plasma cell development remained unclear. Through genetic deletion of the F-actin bundling protein Swiprosin-1/EF-hand domain family member 2 (EFhd2) and by two-photon microscopy, we show that EFhd2 restrains B cell speed in GCs and hapten-specific plasma cell output. Modeling the GC reaction reveals that increasing GC B cell speed promotes plasma cell generation. Lack of EFhd2 also reduces contacts of GC B cells with follicular dendritic cells in vivo. Computational modeling uncovers that both GC output and antibody affinity depend quantitatively on contacts of GC B cells with follicular dendritic cells when B cells migrate more persistently. Collectively, our data explain how GC B cells integrate speed and persistence of cell migration with B cell receptor affinity.en_US
dc.language.isoenen_US
dc.publisherElsevier (CellPress)en_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectB cell receptoren_US
dc.subjectSwiprosin-1/EFhd2en_US
dc.subjectactin cytoskeletonen_US
dc.subjectdifferentiationen_US
dc.subjectfollicular dendritic cellen_US
dc.subjectgerminal centeren_US
dc.subjectmigrationen_US
dc.subjectplasma cellen_US
dc.subjectselectionen_US
dc.subjectsynapseen_US
dc.titleB Cell Speed and B-FDC Contacts in Germinal Centers Determine Plasma Cell Output via Swiprosin-1/EFhd2.en_US
dc.typeArticleen_US
dc.identifier.eissn2211-1247
dc.contributor.departmentBRICS, Braunschweiger Zentrum für Systembiologie, Rebenring 56,38106 Braunschweig, Germany.en_US
dc.identifier.journalCell reportsen_US
dc.source.volume32
dc.source.issue6
dc.source.beginpage108030
dc.source.endpage
refterms.dateFOA2020-11-19T15:12:27Z
dc.source.journaltitleCell reports
dc.source.countryUnited States


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