WAVE1 and WAVE2 have distinct and overlapping roles in controlling actin assembly at the leading edge.
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Authors
Tang, QingSchaks, Matthias
Koundinya, Neha
Yang, Changsong
Pollard, Luther W
Svitkina, Tatyana M
Rottner, Klemens

Goode, Bruce L
Issue Date
2020-07-22
Metadata
Show full item recordAbstract
SCAR/WAVE proteins and Arp2/3 complex assemble branched actin networks at the leading edge. Two isoforms of SCAR/WAVE, WAVE1 and WAVE2, reside at the leading edge, yet it has remained unclear whether they perform similar or distinct roles. Further, there have been conflicting reports about the Arp2/3-independent biochemical activities of WAVE1 on actin filament elongation. To investigate this in vivo, we knocked out WAVE1 and WAVE2 genes, individually and together, in B16-F1 melanoma cells. We demonstrate that WAVE1 and WAVE2 are redundant for lamellipodia formation and motility. However, there is a significant decrease in the rate of leading edge actin extension in WAVE2 KO cells, and an increase in WAVE1 KO cells. The faster rates of actin extension in WAVE1 KO cells are offset by faster retrograde flow, and therefore do not translate into faster lamellipodium protrusion. Thus, WAVE1 restricts the rate of actin extension at the leading edge, and appears to couple actin networks to the membrane to drive protrusion. Overall, these results suggest that WAVE1 and WAVE2 have redundant roles in promoting Arp2/3-dependent actin nucleation and lamellipodia formation, but distinct roles in controlling actin network extension and harnessing network growth to cell protrusion.Citation
Mol Biol Cell. 2020 Sep 15;31(20):2168-2178. doi: 10.1091/mbc.E19-12-0705. Epub 2020 Jul 22.Affiliation
HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.Publisher
American Society for Cell BiologyJournal
Molecular biology of the cellPubMed ID
32697617Type
ArticleLanguage
enEISSN
1939-4586ae974a485f413a2113503eed53cd6c53
10.1091/mbc.E19-12-0705
Scopus Count
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- Creative Commons
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