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dc.contributor.authorHo, Duy-Khiet
dc.contributor.authorChristmann, Rebekka
dc.contributor.authorMurgia, Xabier
dc.contributor.authorde Rossi, Chiara
dc.contributor.authorFrisch, Sarah
dc.contributor.authorKoch, Marcus
dc.contributor.authorSchaefer, Ulrich F
dc.contributor.authorLoretz, Brigitta
dc.contributor.authorDesmaele, Didier
dc.contributor.authorCouvreur, Patrick
dc.contributor.authorLehr, Claus-Michael
dc.date.accessioned2020-11-30T14:28:33Z
dc.date.available2020-11-30T14:28:33Z
dc.date.issued2020-10-19
dc.identifier.citationFront Chem. 2020 Oct 19;8:584242. doi: 10.3389/fchem.2020.584242.en_US
dc.identifier.issn2296-2646
dc.identifier.pmid33195079
dc.identifier.doi10.3389/fchem.2020.584242
dc.identifier.urihttp://hdl.handle.net/10033/622622
dc.description.abstractHepatitis C virus (HCV) has no animal reservoir, infecting only humans. To investigate species barrier determinants limiting infection of rodents, murine liver complementary DNA library screening was performed, identifying transmembrane proteins Cd302 and Cr1l as potent restrictors of HCV propagation. Combined ectopic expression in human hepatoma cells impeded HCV uptake and cooperatively mediated transcriptional dysregulation of a noncanonical program of immunity genes. Murine hepatocyte expression of both factors was constitutive and not interferon inducible, while differences in liver expression and the ability to restrict HCV were observed between the murine orthologs and their human counterparts. Genetic ablation of endogenous Cd302 expression in human HCV entry factor transgenic mice increased hepatocyte permissiveness for an adapted HCV strain and dysregulated expression of metabolic process and host defense genes. These findings highlight human-mouse differences in liver-intrinsic antiviral immunity and facilitate the development of next-generation murine models for preclinical testing of HCV vaccine candidates.en_US
dc.language.isoenen_US
dc.publisherFrontiersen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectdrug deliveryen_US
dc.subjectnanoparticlesen_US
dc.subjectpegylateden_US
dc.subjectprotein-interactionen_US
dc.subjectself-assemblyen_US
dc.subjectsqualeneen_US
dc.subjectsqualenyl derivativesen_US
dc.titleSynthesis and Biopharmaceutical Characterization of Amphiphilic Squalenyl Derivative Based Versatile Drug Delivery Platform.en_US
dc.typeArticleen_US
dc.contributor.departmentHIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1 66123 Saarbrücken, Germany.en_US
dc.identifier.journalFrontiers in chemistryen_US
dc.source.volume8
dc.source.beginpage584242
dc.source.endpage
refterms.dateFOA2020-11-30T14:28:34Z
dc.source.journaltitleFrontiers in chemistry
dc.source.countrySwitzerland


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Except where otherwise noted, this item's license is described as Attribution 4.0 International